YAN Jie

Professor, Mechanobiology Institute, National University of Singapore

phyyj@nus.edu.sg
10-03H
Level 10 T-Lab
National University of Singapore
5A Engineering Drive 1
Singapore 117411

Laboratory website
Yan Jie Group Single Molecule Biophysics

Research Program
Molecular Mechanisms of Mechanobiology Group

Affiliations
Professor, Department of Physics, National University of Singapore

Professor, Centre for BioImaging Sciences, Department of Biological Sciences, National University of Singapore

Recent Research

Pulling biomolecular complexes

MBI scientists demonstrate how the phenomenon of force-induced stabilization of biomolecular complexes can be explained on a one-dimensional energy landscape by incorporating defined transition coordinates.

By Management|Aug 19th, 2019|Categories: Featured Research, Guo S Research, Science Features, Yan Jie Lab, Yefremov Research|Comments Off

Predicting mechanical lifetime

Modelling mechanosense and response

By Management|Sep 3rd, 2018|Categories: Featured Research, Guo S Research, Science Features, Yan Jie Lab|Comments Off

Forcing apart the dual role of formin and profilin

Mechanical and biochemical regulation of actin filament polymerization

By Management|Aug 6th, 2018|Categories: Featured Research, Le SM Research, Science Features, Yan Jie Lab, Yu M Research|Comments Off

12 Next

Yan Jie

Principal Investigator

Research Areas

Stability and Interaction of Biomolecules under Mechanical Constraints

Research Interests

Force-dependent conformations and interactions of nucleic acids and proteins
Mechanosensing of cells at cell-ECM adhesion and cell-cell junction
Mechanisms of chaperone-mediated protein folding
Chromosome packaging, gene regulations, and DNA damage repair

Biography

While being trained in theoretical physics, Dr Jie Yan built a strong interest in studying molecular cell biology using a combination of novel single-molecule biophysical methods and theoretical modeling. Since he joined the Department of Physics of NUS in 2005, he has been leading a group to study DNA-protein interactions and micromechanics of biopolymers. He became a Principal Investigator in MBI in 2009, since then he has been focusing on studies of the effects of mechanical constraints on the stability and interactions of biomolecules.

Recent Publications

Zhang Y, Du J, Liu X, Shang F, Deng Y, Ye J, Wang Y, Yan J, Chen H, Yu M, and Le S. Multi-domain interaction mediated strength-building in human α-actinin dimers unveiled by direct single-molecule quantification. Nat Commun 2024; 15(1):6151. [PMID: 39034324]
Morales-Camilo N, Liu J, Ramírez MJ, Canales-Salgado P, Alegría JJ, Liu X, Ong HT, Barrera NP, Fierro A, Toyama Y, Goult BT, Wang Y, Meng Y, Nishimura R, Fong-Ngern K, Low CSL, Kanchanawong P, Yan J, Ravasio A, and Bertocchi C. Alternative molecular mechanisms for force transmission at adherens junctions via β-catenin-vinculin interaction. Nat Commun 2024; 15(1):5608. [PMID: 38969637]
Sun Y, Liu X, Huang W, Le S, and Yan J. Structural domain in the Titin N2B-us region binds to FHL2 in a force-activation dependent manner. Nat Commun 2024; 15(1):4496. [PMID: 38802383]
Kang M, Otani Y, Guo Y, Yan J, Goult BT, and Howe AK. The focal adhesion protein talin is a mechanically gated A-kinase anchoring protein. Proc Natl Acad Sci U S A 2024; 121(13):e2314947121. [PMID: 38513099]
Liu J, and Yan J. Unraveling the Dual-Stretch-Mode Impact on Tension Gauge Tethers' Mechanical Stability. J Am Chem Soc 2024;. [PMID: 38451494]
Huang W, Liu J, Le S, Yao M, Shi Y, and Yan J. In situ single-molecule investigations of the impacts of biochemical perturbations on conformational intermediates of monomeric α-synuclein. APL Bioeng 2024; 8(1):016114. [PMID: 38435467]
Zhao X, Vogirala VK, Liu M, Zhou Y, Rhodes D, Sandin S, and Yan J. Exploring TRF2-Dependent DNA Distortion Through Single-DNA Manipulation Studies. Commun Biol 2024; 7(1):148. [PMID: 38310140]
Guo Y, Yan J, and Goult BT. Mechanotransduction through protein stretching. Curr Opin Cell Biol 2024; 87:102327. [PMID: 38301379]
Lau MS, Hu Z, Zhao X, Tan YS, Liu J, Huang H, Yeo CJ, Leong HF, Grinchuk OV, Chan JK, Yan J, and Tee W. Transcriptional repression by a secondary DNA binding surface of DNA topoisomerase I safeguards against hypertranscription. Nat Commun 2023; 14(1):6464. [PMID: 37833256]
Fu C, Huang W, Tang Q, Niu M, Guo S, Langenhan T, Song G, and Yan J. Unveiling Mechanical Activation: GAIN Domain Unfolding and Dissociation in Adhesion GPCRs. Nano Lett 2023;. [PMID: 37831892]

Selected Publications

Yuan G, Le S, Yao M, Qian H, Zhou X, Yan J, and Chen H. Elasticity of the Transition State Leading to an Unexpected Mechanical Stabilization of Titin Immunoglobulin Domains. Angew. Chem. Int. Ed. Engl.2017.
You, H., Lattmann, S., Rhodes, D., Yan, J. (2017). “RHAU helicase stabilizes G4 in its nucleotide-free state and destabilizes G4 upon ATP hydrolysis.” Nucleic Acids Research. 46: 206-214.
Yao, M., Goult, B.T., Klapholz, B., Hu, X., Toseland, C.P., Guo, Y., Cong, P., Sheetz, M., and Yan, J. (2016). ” The mechanical response of talin.” Nature Communications, 7: 11966.
Winardhi, R. S., Tang, Q., Chen, J., Yao, M., and Yan, J. (2016). ” Probing Small Molecule Binding to Unfolded Polyprotein Based on its Elasticity and Refolding.” Biophysical Journal, 111(11), 2349-2357.
Cong, P., Dai, L., Chen, H., van der Maarel, J.R.C., Doyle, P.S., and Yan, J. (2015). “Revisiting the Anomalous Bending Elasticity of Sharply Bent DNA.” Biophysical Journal, 109: 2338-2351.
You, H., Wu, J., Shao, F., Yan, J. (2015). “Stability and Kinetics of c-MYC Promoter G-Quadruplexes Studied by Single-Molecule Manipulation.” Journal of the American Chemical Society (Communication), 137: 2424-2427.
Chen, H., Yuan, G., Winardhi, R.S., Yao, M., Popa, I., Fernandez, J.M., Yan, J. (2015). “Dynamics of equilibrium folding and unfolding transitions of titin immunoglobulin domain under constant forces.” Journal of the American Chemical Society, 137: 3540-3546.
You, H., Wu, J., Shao, F., Yan, J. (2015). “Stability and Kinetics of c-MYC Promoter G-Quadruplexes Studied by Single-Molecule Manipulation.” Journal of the American Chemical Society (Communication), 137: 2424-2427.
Zhang, X., Qu, Y., Chen, H., Rouzina, I., Zhang, S., Doyle, P., Yan, J. (2014). “Interconversion between three overstretched DNA structures.” Journal of the American Chemical Society, 136: 16073 -16080.
Yao, M., Qiu, W., Liu, R. , Efremov, A.K., Cong, P., Seddiki, R., Payre, M., Lim, C.T., Ladoux, B., Me`ge, R.M., and Yan, J. (2014). “Force-dependent conformational switch of alpha-catenin controls vinculin binding.” Nature Communications, 5:4525.