YAN Jie

Professor, Mechanobiology Institute, National University of Singapore

phyyj@nus.edu.sg
10-03H
Level 10 T-Lab
National University of Singapore
5A Engineering Drive 1
Singapore 117411

Laboratory website
Yan Jie Group Single Molecule Biophysics

Research Program
Molecular Mechanisms of Mechanobiology Group

Affiliations
Professor, Department of Physics, National University of Singapore

Professor, Centre for BioImaging Sciences, Department of Biological Sciences, National University of Singapore

Yan Jie

Principal Investigator

Research Areas

Stability and Interaction of Biomolecules under Mechanical Constraints

Research Interests

• Force-dependent conformations and interactions of nucleic acids and proteins
• Mechanosensing of cells at cell-ECM adhesion and cell-cell junction
• Mechanisms of chaperone-mediated protein folding
• Chromosome packaging, gene regulations, and DNA damage repair

Biography

While being trained in theoretical physics, Dr Jie Yan built a strong interest in studying molecular cell biology using a combination of novel single-molecule biophysical methods and theoretical modeling. Since he joined the Department of Physics of NUS in 2005, he has been leading a group to study DNA-protein interactions and micromechanics of biopolymers. He became a Principal Investigator in MBI in 2009, since then he has been focusing on studies of the effects of mechanical constraints on the stability and interactions of biomolecules.

Recent Publications

Selected Publications

1. Yuan G, Le S, Yao M, Qian H, Zhou X, Yan J, and Chen H. Elasticity of the Transition State Leading to an Unexpected Mechanical Stabilization of Titin Immunoglobulin Domains. Angew. Chem. Int. Ed. Engl.2017.
2. You, H., Lattmann, S., Rhodes, D., Yan, J. (2017). “RHAU helicase stabilizes G4 in its nucleotide-free state and destabilizes G4 upon ATP hydrolysis.” Nucleic Acids Research. 46: 206-214.
3. Yao, M., Goult, B.T., Klapholz, B., Hu, X., Toseland, C.P., Guo, Y., Cong, P., Sheetz, M., and Yan, J. (2016). ” The mechanical response of talin.” Nature Communications, 7: 11966.
4. Winardhi, R. S., Tang, Q., Chen, J., Yao, M., and Yan, J. (2016). ” Probing Small Molecule Binding to Unfolded Polyprotein Based on its Elasticity and Refolding.” Biophysical Journal, 111(11), 2349-2357.
5. Cong, P., Dai, L., Chen, H., van der Maarel, J.R.C., Doyle, P.S., and Yan, J. (2015). “Revisiting the Anomalous Bending Elasticity of Sharply Bent DNA.” Biophysical Journal, 109: 2338-2351.
6. You, H., Wu, J., Shao, F., Yan, J. (2015). “Stability and Kinetics of c-MYC Promoter G-Quadruplexes Studied by Single-Molecule Manipulation.” Journal of the American Chemical Society (Communication), 137: 2424-2427.
7. Chen, H., Yuan, G., Winardhi, R.S., Yao, M., Popa, I., Fernandez, J.M., Yan, J. (2015). “Dynamics of equilibrium folding and unfolding transitions of titin immunoglobulin domain under constant forces.” Journal of the American Chemical Society, 137: 3540-3546.
8. You, H., Wu, J., Shao, F., Yan, J. (2015). “Stability and Kinetics of c-MYC Promoter G-Quadruplexes Studied by Single-Molecule Manipulation.” Journal of the American Chemical Society (Communication), 137: 2424-2427.
9. Zhang, X., Qu, Y., Chen, H., Rouzina, I., Zhang, S., Doyle, P., Yan, J. (2014). “Interconversion between three overstretched DNA structures.” Journal of the American Chemical Society, 136: 16073 -16080.
10. Yao, M., Qiu, W., Liu, R. , Efremov, A.K., Cong, P., Seddiki, R., Payre, M., Lim, C.T., Ladoux, B., Me`ge, R.M., and Yan, J. (2014). “Force-dependent conformational switch of alpha-catenin controls vinculin binding.” Nature Communications, 5:4525.