Anne BEGHIN

Research Assistant Professor, Mechanobiology Institute, National University of Singapore

Facility Manager, Microscopy Core

mbianne@nus.edu.sg
Level 9 T-Lab
National University of Singapore
5A Engineering Drive 1
Singapore 117411

Anne Beghin

Research Assistant Professor, Facility Manager Microscopy Core

Research Interests

– Mechanobiology, cancer and immunology research
– Organoids, Organ-on-chip, 3D bioprinter
– Microscopy, AI, image analysis
– Microgravity and Space environnements (regolith, radiation, microgravity) effect on human tissue homeostasis

SIMBA – SIngapore Microscopy and Bioimage Analysis
Technology Innovation for Mechanobiology

Selected Publications

  • Ong H, …Beghin A. (last and corresponding author). Digitalized organoids: integrated pipeline for 3D high-speed analysis of organoid structures using multilevel segmentation and cellular topology. Under revision and BiorXiv, 2024. DOI: 10.1101/2023.11.08.566158
  • Beghin A (corresponding author), et al. Automated high-speed 3D imaging of organoid cultures with multi-scale phenotypic quantification. Nat Methods, July, 2022. (Cover)DOI: 10.1038/s41592-022-01508-0
  • Grenci G, … Beghin A. (last and corresponding author). A High-Throughput Platform for Culture and 3D Imaging of Organoids. Jove, nov 2023. DOI: 10.3791/64405
  • Beghin A, et al. High content 3D imaging method for quantitative characterization of organoid development and phenotype. BiorXiv, 2021. DOI: 10.1101/2021.03.26.437121
  • Levet F, …, Beghin A, et al. A tessellation-based colocalization analysis approach for single-molecule localization microscopy. Nat Methods. 2019 May. DOI: 10.1038/s41467-019-10007-4
  • Beghin A, et al. Localization-based super-resolution imaging meets high-content screening. Nat Methods. 2017 Dec. DOI: 10.1038/nmeth.4486
  • Levet F, …, Beghin A, et al. SR-Tesseler: a method to segment and quantify localization-based super-resolution microscopy data. Nat Methods. 2015 Nov. DOI: 10.1038/nmeth.3579
  • Woeffler-Maucler C, Beghin A, et al. Automated immunohistochemical method to quantify neuronal density in brain sections: application to neuronal loss after status epilepticus. J Neurosci Methods. 2014. DOI: 10.1016/j.jneumeth.2014.01.009
  • Prudent R, …, Beghin A, et al. Pharmacological inhibition of LIM kinase stabilizes microtubules and inhibits neoplastic growth. Cancer Res. 2012 Sep. DOI: 10.1158/0008-5472.CAN-11-3342
  • Degot S, …, Beghin A, et al. Improved visualization and quantitative analysis of drug effects using micropatterned cells. J Vis Exp. 2010 Dec. DOI: 10.3791/2514
  • Simon B, …, Beghin A, et al. High-resolution tomographic diffractive microscopy of biological samples. J Biophotonics. 2010 Jul. DOI: 10.1002/jbio.200900094
  • Beghin A, et al. ADP ribosylation factor like 2 (Arl2) regulates breast tumor aggressivity in immunodeficient mice. PLoSOne. 2009 Oct. DOI: 10.1371/journal.pone.0007478
  • Beghin A, et al. Dysregulation of ribosome biogenesis and translational capacity is associated with tumor progression of human breast cancer cells. PLoS One. 2009 Sep. DOI: 10.1371/journal.pone.0007147
  • Beghin A, et al. Expression of Arl2 is associated with p53 localization and chemosensitivity in a breast cancer cell line. Cell Cycle. 2008 Oct. DOI: 10.4161/cc.7.19.6777
  • Beghin A, et al. Tubulin folding pathways: implication in the regulation of microtubule dynamics. Curr Cancer Drug Targets. 2007 Dec. DOI: 10.2174/156800907783220426
  • Beghin A, et al. ADP ribosylation factor like 2 (Arl2) protein influences microtubule dynamics in breast cancer cells. Exp Cell Res. 2007 Feb. DOI: 10.1016/j.yexcr.2006.10.024
  • Galmarini CM, …, Beghin A, et al. A p21/WAF1 mutation favors the appearance of drug resistance to paclitaxel in human noncancerous epithelial mammary cells. Int J Cancer. 2006 Jul. DOI: 10.1002/ijc.21770

Patent Filed

  • Beghin A, et al. A Cell Culture Plate for Conducting 3D Cell Culture And A Method of Fabricating the Same. No. PCT/SG2021/050082. 2021.

Recent Publications

  1. Ong HT, Karatas E, Poquillon T, Grenci G, Furlan A, Dilasser F, Mohamad Raffi SB, Blanc D, Drimaracci E, Mikec D, Galisot G, Johnson BA, Liu AZ, Thiel C, Ullrich O, , Racine V, and Beghin A. Digitalized organoids: integrated pipeline for high-speed 3D analysis of organoid structures using multilevel segmentation and cellular topology. Nat Methods 2025;. [PMID: 40369245]
  2. Verstegen MMA, Coppes RP, Beghin A, De Coppi P, Gerli MFM, de Graeff N, Pan Q, Saito Y, Shi S, Zadpoor AA, and van der Laan LJW. Clinical applications of human organoids. Nat Med 2025;. [PMID: 39901045]
  3. Grenci G, Dilasser F, Mohamad Raffi SB, Marchand M, Suryana M, Sahni G, Viasnoff V, and Beghin A. A High-Throughput Platform for Culture and 3D Imaging of Organoids. J Vis Exp 2022;(188). [PMID: 36314813]
  4. Beghin A, Grenci G, Sahni G, Guo S, Rajendiran H, Delaire T, Mohamad Raffi SB, Blanc D, de Mets R, Ong HT, Galindo X, Monet A, Acharya V, Racine V, Levet F, Galland R, Sibarita J, and Viasnoff V. Automated high-speed 3D imaging of organoid cultures with multi-scale phenotypic quantification. Nat Methods 2022;. [PMID: 35697835]
  5. Boulan B, Beghin A, Ravanello C, Deloulme J, Gory-Fauré S, Andrieux A, Brocard J, and Denarier E. AutoNeuriteJ: An ImageJ plugin for measurement and classification of neuritic extensions. PLoS ONE 2020; 15(7):e0234529. [PMID: 32673338]