Stuti K DESAI
Alumni, Mechanobiology Institute, National University of Singapore
Seeking Postdoctoral Research Fellow in the research group of Dr. Tsuyoshi Hirashima at the Mechanobiology Institute, NUS ( Cloned )
We are seeking to recruit a Postdoctoral Research Fellow in the Collective Cell Dynamics in Mammalian Development and Reproduction Laboratory at the Mechanobiology Institute, NUS.
MBI Researchers Identify a Signalling Pathway in Stiff Ovarian Tissue Affecting Egg Maturation
A joint study between the Li and Young Labs at MBI, together with A*STAR's Institute of Molecular and Cell Biology, pinpoints the molecular switch responsible for matrix stiffness in ageing ovaries, offering an alternative way to treat age-related infertility without having to reverse stiffness.
MBI to host EMBO Workshop on Cell and Tissue Mechanics of Aging and Regeneration
Organised and hosted by MBI, this EMBO Workshop will be held on 21-24 September 2027 at the Shaw Foundation Alumni House, NUS. Please click on the link to register your interest: https://coming-soon.embo.org/gw27-14
Seeking Research Fellow in Cardiac Organoids and Microphysiological Systems at the Mechanobiology Institute, NUS
The laboratory of Prof. Hanry Yu seeks to to recruit a Research Fellow in Cardiac Organoids and Microphysiological Systems at the Mechanobiology Institute, NUS.
Xiangfu Guo
Research Fellow, Yan Jie Group
Chu Đặng Khánh Hoà
Research Assistant, Dye & Michelot Groups
Stuti K Desai
Alumni 2020
Principal Investigator
Research Interests
Molecular Mechanisms of Mechanobiology
A fundamental trait shared amongst many mammalian pathogens is their ability to exist in different forms in order to survive and replicate in often fluctuating host and non-host environments. Two important gut pathogens, Salmonella Typhimurium and Salmonella Typhi, which cause gastroenteritis and typhoid fever, respectively, survive inside host cells by activation of a specific class of virulence genes. Salmonellae also develop to form hardy multi-cellular communities, called biofilms, on host tissues for successful survival and persistence in asymptomatic carriers. An active area of research focuses on understanding how the genetic program is regulated in coordination with environmental changes to evoke lifestyle changes. We recently discovered that SsrB, the transcriptional regulator of virulence genes, has the additional function of inducing biofilm formation by activating expression of the central biofilm regulator, CsgD. Generally, specific environmental changes activate a membrane-bound kinase, which in turn transfers the phosphoryl group to a transcriptional regulator in order to regulate virulence gene transcription. But, we discovered that even without phosphorylation, SsrB retains the ability to bind DNA and relieve the H-NS-mediated repressive effect on csgD transcription. SsrB is thus a molecular switch which drives the twin lifestyles of Salmonella Typhimurium; as SsrB~P, when phosphorylated by an actived SsrA kinase, it activates around 30 virulence genes required for survival within the host; and as SsrB, in the case when SsrA is inactive or absent, it counters transcriptional silencing at csgD to form biofilms.
For this study, we utilized the highly collaborative atmosphere at MBI and studied the behavior of the activator, SsrB, and the repressor, H-NS, at the csgD regulatory site using single molecule approaches with Prof. Yan Jie’s group. Going forward, I want to build on the diverse expertise at MBI and other groups in the region to extend these findings and study the specific mechanisms of lifestyle changes in more aggressive strains of Salmonella and validate the underlying signaling pathways in heterologous hosts as model systems.
Research Areas
Bacterial gene regulation, Biofilms, Host-pathogen interactions and Virulence strategies of Salmonellae
Biography
Dr. Stuti Desai joined the group of Prof. Linda Kenney at the Mechanobiology Institute, in 2012, after obtaining her doctorate from the Indian Institute of Science, Bangalore, India, under the guidance of Prof. Subramony Mahadevan. Her graduate studies involved deciphering the regulatory mechanisms that maintain the beta-glucoside utilization systems in a silent state in commensal as well as pathogenic gut bacteria. Prior to that, she studied Biochemistry for her Masters degree at the Maharaja Sayajirao University of Baroda, Baroda, India.
Recent Publications
- Desai SK, and Kenney LJ. Switching Lifestyles Is an in vivo Adaptive Strategy of Bacterial Pathogens. Front Cell Infect Microbiol 2019; 9:421. [PMID: 31921700]
- Desai SK, Padmanabhan A, Harshe S, Zaidel-Bar R, and Kenney LJ. Salmonella biofilms program innate immunity for persistence in Caenorhabditis elegans. Proc. Natl. Acad. Sci. U.S.A. 2019;. [PMID: 31160462]
- Dykas MM, Desai SK, Patra A, Motapothula MR, Poddar K, Kenney LJ, and Venkatesan T. Identification of Biofilm Inhibitors by Screening Combinatorial Libraries of Metal Oxide Thin Films. ACS Appl Mater Interfaces 2018;. [PMID: 29553712]
- Desai SK, and Kenney LJ. To ∼P or Not to ∼P? Non-canonical Activation by Two-Component Response Regulators. Mol. Microbiol. 2016;. [PMID: 27656860]
- Desai SK, Winardhi RS, Periasamy S, Dykas MM, Jie Y, and Kenney LJ. The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing. Elife 2016; 5. [PMID: 26880544]
