Anand Pratap SINGH
MBI Alumni, Mechanobiology Institute, National University of Singapore
mbiaps@nus.edu.sg
Level 10 T-Lab
National University of Singapore
5A Engineering Drive 1
Singapore 117411
So-SPIM-FCS
A novel tool for imaging nuclear protein dynamics
Anand Pratap Singh
MBI Alumni, June 2017
Principal Investigator
Research Interests
Mechanotransduction in Tissues Group
My research focuses on developing new quantitative bioimaging tools to understand spatio-temporal protein dynamics and protein-protein interaction on a single cell level. During my graduate study, I focused on building, characterization and various applications of the light sheet based fluorescence microscope. Light sheet based microscope also known as single plane illumination microscope (SPIM) is an imaging based quantitative method and has been successfully combined with FC(C)S to quantify concentrations, molecular dynamics, and interactions in live cells.
Currently, I am working with Assist. Prof. Timothy Saunders, to understand the complex spatio-temporal behavior of tissue birth and death during early embryogenesis of Drosophila.
Current position
Mechanobiology Institute, Department of Biological Sciences, NUS, Research assistant with Assist. Prof. Timothy Saunders. I will be also working with Prof. Dr. G.V Shivashankar at MBI, NUS.
Education
Graduate Studies 2010-2014, Ph.D advisor: Assist. Prof. Dr. Thorsten Wohland, Departments of Biological Sciences and Chemistry and Centre for Bio-Imaging Sciences, NUS, Singapore.
Master of Science 2007-2009, Physical Chemistry, Organic Chemistry, and Inorganic Chemistry; Banaras Hindu University, Varanasi, India.
Bachelor of Science 2003-2006, Physics, Chemistry and Mathematics; B.S.N.V. P.G College, University of Lucknow, Lucknow, India.
List of publications
Anand P. Singh, Thorsten Wohland; Towards the measurement of molecular interactions in live tissues by single plane illumination microscopy fluorescence correlation spectroscopy (SPIM-FCS), Micros. Anal., July 2012.
Anand P. Singh, Jan W. Krieger, Jan Buchholz, Edoardo Charbon, Jörg Langowski, and Thorsten Wohland; The performance of 2D array detectors for light sheet based fluorescence correlation spectroscopy, Opt Express. 2013.
Jan W. Krieger, Anand P. Singh, Christoph S. Garbe, Thorsten Wohland, and Jörg Langowski; Dual-color fluorescence cross-correlation spectroscopy on a single plane illumination microscope (SPIM-FCCS), Opt Express, 2014.
Anand P. Singh, and Thorsten Wohland; Applications of imaging fluorescence correlation spectroscopy, Curr. Opin. Chem. Biol., 2014.
Conferences and workshops
Fluorescence Correlation Spectroscopy (FCS) and related methods, Oct. 2010.
Platform presentation- “The third Light Sheet Microscopy Workshop”, October, Toulouse, France, Oct. 2011.
Poster presentation-“Focus on Microscopy”, Singapore, April 2012.
Poster Presentation-“2nd Southeast Asian Course on F-techniques”, Singapore, July 2012.
Platform presentation (lab visit 2 weeks)-“German Cancer Research Centre (DKFZ)”, Biophysics of Macromolecules (B040), Heidelberg, Oct. 2011.
Poster presentation-“7th Singapore International Chemistry Conference (SICC7)”, Singapore, Dec. 2012.
Poster presentation, “Biophysical Society 57th Annual Meeting”, Phil., USA, Feb. 2013.
Platform presentation, “The fifth Light Sheet Microscopy Workshop”, New York, 2013.
Poster presentation, “Single Protein Dynamics in cellulo: Spatio-temporal, Structural and Quantitative Analyses (SPDC2014)”, Japan, 2014.
Teaching and mentoring
Practical “Advanced Experiments in Analytical & Physical Chemistry, CM3292”, Semester 1, 2010-2011.
Practical “Advanced Experiments in Analytical & Physical Chemistry, CM3292”, Semester 2, Teaching Assessment, 2010-2011.
Practical “Advanced Experiments in Analytical & Physical Chemistry, CM3292”, Special Term, 2010-2011.
SPIM-FCS sample mounting, measurements and data analysis “Genetics, Genomics and Imaging in Medaka and Zebrafish Workshop”, July 2012.
SPIM-FCS sample mounting, measurements and data analysis, “Mechanobiology of Chromatin and Transcription Workshop”, November 2012.
Fellowships and awards
Graduate Aptitude Test in Engineering (GATE), Chemistry, percentile 99.81, India, 2009.
Junior Research Fellowship, Council of Scientific & Industrial Research (JRF-CSIR), Chemical Sciences, India, 2009.
National University of Singapore graduate fellowship, Singapore, 2010.
Conference funding, Department of Chemistry, NUS, Singapore, 2013.
Student Research Achievement Award (SARA), 57th Biophysical Society Meeting, USA, 2013.
SPDC Japan conference funding, 2014.
Recent Publications
- Rupprecht J, Ong KH, Yin J, Huang A, Dinh H, Singh AP, Zhang S, Yu W, and Saunders TE. Geometric constraints alter cell arrangements within curved epithelial tissues. Mol. Biol. Cell 2017;. [PMID: 28978739]
- Singh AP, Galland R, Finch-Edmondson ML, Grenci G, Sibarita J, Studer V, Viasnoff V, and Saunders TE. 3D Protein Dynamics in the Cell Nucleus. Biophys. J. 2017; 112(1):133-142. [PMID: 28076804]
- Krieger JW, Singh AP, Bag N, Garbe CS, Saunders TE, Langowski J, and Wohland T. Imaging fluorescence (cross-) correlation spectroscopy in live cells and organisms. Nat Protoc 2015; 10(12):1948-74. [PMID: 26540588]
