Melvino SIN

Senior Lab Executive, Mechanobiology Institute, National University of Singapore

melvino@nus.edu.sg
Level 10 T-Lab
National University of Singapore
5A Engineering Drive 1
Singapore 117411

Melvino Sin

Senior Laboratory Executive, SIMBA

Principal Investigator

Anne Beghin

Research Areas

Light Microscopy – STORM Super-Resolution, Automated Slide Scanning.

Qualifications

  • MSc Biomedical Engineering, Nanyang Technological University, Singapore (NTU)
  • BSc Biomedical Sciences, University of Western Australia (UWA)
  • Dip Pharmaceutical Sciences, Temasek Polytechnic

Biography

Melvino obtained his BSc in Biomedical Science from UWA, and his MSc in Biomedical Engineering from NTU. He started his career in 2010 at IMCB, A*STAR, researching on EVL cells and neurulation of the neural tube in Zebrafish in Vladimir Korzh’s Fish Developmental Biology Lab and subsequently took charge of running the Central Imaging Facility in 2012. In 2015, Melvino moved to NTU for 2 years to manage the SCELSE Advanced Biofilm Imaging Facility before resuming his former post in IMCB, A*STAR Central Imaging Facility. In 2019, he was headhunted by Andor Technology and was deeply immersed in the commercial sector. He organized the Andor Academy hosted in MBI in Aug 2019, where it provided an enriching learning experience to many MBI staff and students.

Melvino joined MBI’s Microscopy Core (SIMBA) on 1 Sep 2021, where he is in-charge of the STORM Super-Resolution systems, Olympus Live/EZ systems, and AxioScan slide scanner. Above and beyond microscopy and conducting trainings for these systems, he is also SIMBA’s Safety Lead and is responsible for managing N2 and N3 licence applications and record-keeping.

Technical Skills

  • Spinning-Disk confocal microscopy
  • Selective Plane Illumination Microscopy (SPIM)
  • Photoactivated Localization Microscopy (PALM)
  • Direct-Stochastic Optical Reconstruction Microscopy (dSTORM)
  • Super-resolution Structured Illumination Microscopy (SR-SIM)
  • Total Internal Reflection Fluorescence (TIRF) microscopy
  • F-techniques (FRAP/FRET)
  • Lambda scanning (also known as emission fingerprinting)
  • Transmitted Light techniques – Bright-field, DIC, Phase Contrast imaging
  • High content imaging
  • Live-cell imaging

Other Professional Involvement

  • Member of SingaScope
  • Lab Safety Lead
  • Biological Spill Responder
  • Chemical Spill Responder
  • Image analysis & processing

Selected Publications

  • Yang, S., Emelyanov, A., You, MS. et al. Camel regulates development of the brain ventricular system. Cell Tissue Res 383, 835–852 (2021). https://doi.org/10.1007/s00441-020-03270-1
  • Kondrychyn I, Teh C, Sin M, Korzh V (2013) Stretching Morphogenesis of the Roof Plate and Formation of the Central Canal. PLoS ONE 8(2): e56219. https://doi.org/10.1371/journal.pone.0056219
  • G. Stewart, D. Spagnolo, M. E. Polomska, M. Sin, M. Karimi, L. J. Abraham, Synthesis and TNF expression inhibitory properties of new thalidomide analogues derived via Heck cross coupling. Bioorg. Med. Chem Letts. 2007, 17, 5819. https://doi.org/10.1016/j.bmcl.2007.08.042