MBI PhD Oral Defense

Time: 2pm-3pm
Date: Tuesday, 5 December 2017
Venue: MBI, level 5 meeting rooms
Supervisor: Prof Kanchanawong, Pakorn

Topological Analysis of Cytoskeletal Networks from Single-Molecule Localization Microscopy  Images

by Zhang Zhen, Kanchanawong Group

The cytoskeletons are fundamental structural elements of the cells, playing essential roles in the maintenance and regulation of cell shape, cell mechanics, and numerous cell behavioral programmes. Complex networks of the cytoskeletons permeate the cell interior and their architecture is a critical physical attribute governing their biological functions. However, several challenges remain in the quantitative understanding of the cytoskeletons. In this dissertation, I addressed these by an integrated approach combining super-resolution microscopy with advanced computational analysis. First, a systematic optimization of single-molecule localization super-resolution microscopy was carried out. This enabled the visualization of the cytoskeletons at nanoscale resolution, revealing highly complex organization. Second, novel computational approaches were developed for the quantitative analysis of the highly entangled cytoskeletal networks from super-resolution images. This enabled automated high-throughput analysis of the cytoskeletal filaments from complex images. These advances were demonstrated in the studies of microtubule network organization downstream of the small GTPase Rac1, and the Sertoli cell cortical F-actin network organization downstream of the actin polymerization factors, mDia1 and mDia3. 

*All except panel are excused for closed-door examination*