Michael SHEETZ
Founding Director and Collaborator, Mechanobiology Institute, National University of Singapore
Affiliations
Professor, Department of Biochemistry & Molecular Biology, UTMB Health
What is Rho-dependent IL-2 receptor endocytosis?
What is Rho-dependent IL-2 receptor endocytosis? Internalization of transmembrane receptors was thought to occur exclusively via clathrin-mediated endocytosis (CME). However, the discovery of clathrin-independent endocytic routes, in particular, the pathway that mediates the internalization [...]
What is Fast Endophilin-Mediated Endocytosis (FEME)?
What is Fast Endophilin-Mediated Endocytosis (FEME)? FEME is a novel clathrin-independent endocytic pathway, regulated by the BAR domain protein endophilin, where tubulo-vesicular carriers form within seconds at the plasma membrane upon activation of specific [...]
How are clathrin-coated vesicles transported?
How are clathrin-coated vesicles transported? Different cytoskeletal networks have been implicated in the transport of clathrin-coated vesicles (CCVs). In yeast, the actin cytoskeleton traffics dissociated CCVs [1], whilst in mammals the microtubule network transports [...]
How are clathrin-coated vesicles uncoated?
How are clathrin-coated vesicles uncoated? Uncoating is the process by which clathrin is removed from clathrin-coated vesicles (CCVs). In mammals, this ATP dependent process is driven by the 70kDa molecular chaperone ‘Heat shock cognate [...]
How does scission of the clathrin-coated vesicle occur?
How does scission of the clathrin-coated vesicle occur? As mentioned, in mammalian CME the GTPase dynamin is believed to play important roles in invagination and clathrin-coated pit (CCP) maturation [1]. Its binding partners endophilin [...]
Michael Sheetz
Collaborator
Research Areas
Cell migration, cell-cell and cell-substrate interaction
Research Interests
The morphology of cells, organs and whole organisms is determined by the generation of forces on the immediate environment, which is either extracellular matrix or adjacent cells. We are currently engaged in studies to understand the detailed molecular mechanisms involved in a variety of phenomena from cancer metastasis to brain function. Further, we are developing several new tools and protocols for measuring cell forces at the molecular level, which are revealing many new aspects of how cells can both generate and respond to external forces. We have an effort underway to define quantitatively the steps involved in cell adhesion to and spreading on a matrix-coated surface. Using a variety of cell lines that are missing proteins in various motility pathways, we are determining the quantitative changes in the spreading process. This will enable us to generate a detailed model of the process of spreading that will be a model for further studies of how cells differentiate, regenerate tissues or metastasize.
Biography
Hailing from Columbia University, Prof Michael Sheetz has more than 40 years’ experience in the biomedical field. Introduced to Singapore by Prof Hew Choy Leong of the National University of Singapore’s Department of Biological Sciences, Prof Sheetz was sought to lead an RCE project. This resulted in a two-year effort to organise and submit a proposal on Mechanobiology where Prof Sheetz set the theme and direction of the Mechanobiology Institute (MBI). As Founding Director of the MBI, he led the institute for 10 years and built it into a world-leading research centre in the field of mechanobiology.
Education
PhD California Institute of Technology
Recent Publications
- Jain K, Kishan K, Minhaj RF, Kanchanawong P, Sheetz MP, and Changede R. Immobile Integrin Signaling Transit and Relay Nodes Organize Mechanosignaling through Force-Dependent Phosphorylation in Focal Adhesions. ACS Nano 2025;. [PMID: 39760672]
- Lin S, Changede R, Farrugia AJ, Bershadsky AD, Sheetz MP, Prost J, and Rupprecht J. Membrane Tilt Drives Phase Separation of Adhesion Receptors. Phys Rev Lett 2024; 132(18):188402. [PMID: 38759206]
- Jain K, Minhaj RF, Kanchanawong P, Sheetz MP, and Changede R. Nano-clusters of ligand-activated integrins organize immobile, signalling active, nano-clusters of phosphorylated FAK required for mechanosignaling in focal adhesions. bioRxiv 2024;. [PMID: 38464288]
- Jain K, Pandey A, Wang H, Chung T, Nemati A, Kanchanawong P, Sheetz MP, Cai H, and Changede R. TiO2 Nano-Biopatterning Reveals Optimal Ligand Presentation for Cell-Matrix Adhesion Formation. Adv Mater 2024;:e2309284. [PMID: 38340044]
- Jain K, Lim KYE, Sheetz MP, Kanchanawong P, and Changede R. Intrinsic self-organization of integrin nanoclusters within focal adhesions is required for cellular mechanotransduction. bioRxiv 2023;. [PMID: 38045378]
- Oh D, Liu X, Sheetz MP, and Kenney LJ. Small, Dynamic Clusters of Tir-Intimin Seed Actin Polymerization. Small 2023;:e2302580. [PMID: 37649226]
- Qin R, Melamed S, Yang B, Saxena M, Sheetz MP, and Wolfenson H. Tumor Suppressor DAPK1 Catalyzes Adhesion Assembly on Rigid but Anoikis on Soft Matrices. Front Cell Dev Biol 2022; 10:959521. [PMID: 35927990]
- Jain K, Kanchanawong P, Sheetz MP, Zhou X, Cai H, and Changede R. Ligand functionalization of titanium nanopattern enables the analysis of cell-ligand interactions by super-resolution microscopy. Nat Protoc 2022;. [PMID: 35896742]
- Toh PJY, Lai JKH, Hermann A, Destaing O, Sheetz MP, Sudol M, and Saunders TE. Optogenetic control of YAP cellular localisation and function. EMBO Rep 2022;:e54401. [PMID: 35876586]
- Oh D, Chen Z, Biswas KH, Bai F, Ong HT, Sheetz MP, and Groves JT. Competition for shared downstream signaling molecules establishes indirect negative feedback between EGFR and EphA2. Biophys J 2022;. [PMID: 35430415]
Lab Members
Sree Vaishnavi Sundararajan
Alumni, Grenci Group
Mona Suryana
Research Associate, Grenci Group
Jegan Vishnuwardhana Shanmugarajah
Research Assistant, Grenci Group
Breaking cell symmetry
A force driven mechanism for establishing cell polarity
MBI welcomes students from Huazhong University of Science and Technology, China
On August 16th, a group of 13 students from the Huazhong University of Science and Technology (HUST), China visited the Mechanobiology Institute (MBI), National University of Singapore to learn more about scientific research in Singapore.
Yuan Luezhen
Alumni, Shivashankar Group
Dasvit Shetty
PhD Student, Class of August 2017, Yan Jie Group, Kenney Group
Toh Jia Ying Pearlyn
PhD Student, Class of August 2017, Saunders Group
Tricia Loo Yi Jun
PhD Student, Class of August 2017, Saunders Group
MBI welcomes our incoming class of August 2017
In August, 2017, MBI welcomes our latest incoming class of doctoral students, ten highly qualified candidates from schools both here in Singapore and abroad
Xu Yumeng
Alumni Class of August 2017, Yu Group
Paramasivam Kathirvel
Facility Manager, Shivashankar Group
