Gianluca GRENCI
Research Assistant Professor, Department of Biomedical Engineering, National University of Singapore
Facility Manager, Nano and Microfabrication Core
mbigg@nus.edu.sg
Level 10 T-Lab
National University of Singapore
5A Engineering Drive 1
Singapore 117411
How does the tubulin complex assemble?
How does the tubulin complex assemble? Microtubules are made up of repeating units of α/β- tubulin heterodimers, which are assembled on a γ-tubulin ring complex (a complex of γ-tubulin and other protein components), during [...]
What is microtubule dynamic instability?
What is microtubule dynamic instability? In most cell types, thirteen protofilaments associate laterally to form a microtubule. In a few cases microtubules contain more or fewer protofilaments [1]. Numerous interactions between the subunits give [...]
What is the role of microtubules in mechanotransduction?
What is the role of microtubules in mechanotransduction? Microtubules exist in all cells, however their influence in the mechanotransduction of mechanical stimuli has been described at length in cardiac striated muscle [1]. Mechanical stimuli [...]
What are Contractile Fibers?
What are Contractile Fibers? Certain myosin isoforms (i.e. myosin II) form bipolar assemblies via the extended coiled-coil domains in the heavy chains (see also “thick filaments”). Actin “thin filaments” with opposite polarity associate with [...]
What is Actomyosin?
What is Actomyosin? Actomyosin refers to the actin-myosin complex that forms within the cytoskeleton. Actomyosin is inherently contractile, with the myosin motor protein able to pull on actin filaments. This property gives rise to [...]
What are Blebs?
What are Blebs? Blebs are blister-like protrusions that occur at the cell surface (reviewed in [1]). Blebs form, and function, in a series of defined steps. They typically grow to a length of around [...]
Gianluca Grenci
Research Assistant Professor, Facility Manager
Research Interests
Microfluidics, Micro-optical systems for live cell imaging
Our laboratory is primarily interested in the application of micro/nano fabrication technology to biological science. We exploit standard and advanced micro-fabrication tools in order to design and produce systems and devices for cell culturing and imaging. Examples of such devices are: topographically and/or chemically micro-textured environments, microfluidic devices, micro-optical systems and more.
We are also interested in developing microfluidic devices for FTIR spectromicroscopy of living cells. FTIR is an imaging technique that is intrinsically label-free and requires minimal sample preparation; when coupled with microscopy and high brilliance IR sources it allows the acquisition of chemical maps at a resolution which is diffraction limited. Absorption of IR photons induces very low or no damage at all, therefore it is in principle possible to observe for prolonged time the behaviour of living cells. Our research activity is intended to develope microfludic platforms suitable for FTIR (key parameters are optical transparency and low IR absorption) while keeping cells alive and healthy; a beneficial feature provided by micro-fabrication approach is the possibility to control of the chemical environment at the micro-scale.
Research Areas
Micro/nano engineering, microfluidic, FTIR
Biography
Dr Gianluca Grenci joined MBI in 2012 as a research fellow and head of the Micro Fabrication Core facility. Previously he was employed at the LILIT micro/nano fabrication group (IOM-CNR, Trieste, IT) for a total of 6 years, during which he was mainly involved in the design and fabrication of microfluidic devices for synchrotron-light related spectroscopic techniques, such as SAXS and FTIR. He thus developed extensive practical knowledge on all the major lithographic technologies (UV and EB lithography, wet/dry etching, soft-lithography, thin films deposition), plus some less usual and/or more advanced technique, such as X-ray Lithography and LIGA.
He did his PhD in the field of applied superconductivity, in a project aimed to develop a current cryo-comparator (CCC) using high critical temperature superconductors of the cuprate family (YBCO) in the form of a thick film deposited onto a large area, complex shaped silver substrate.
Education
PhD Polytechnic of Torino, DISPEA
Recent Publications
- Zhang Z, Canela A, Kurisu J, Zou P, Kawaue T, Nakazawa N, Takeda N, Saeki M, Utsunomiya M, Bilgic M, Ishidate F, Grenci G, Furuta T, Kishi Y, Sasanuma H, and Kengaku M. Confined migration induces non-lethal DNA damage in developing neurons. Nature 2026;. [PMID: 42310452]
- Gandin A, Torresan V, Panciera T, Grenci G, Vanni G, Citron A, Marchionni M, Battilana G, Pelosin M, Busetto R, Piccolo S, and Brusatin G. Flexible high-resolution ECM micropatterning. Nat Protoc 2026;. [PMID: 42129485]
- Dunsing-Eichenauer V, Hummert J, Chardès C, Schönau T, Guignard L, Galland R, Grenci G, Tillmann M, Koberling F, Nock C, Sibarita J, Viasnoff V, Antolovic IM, Erdmann R, and Lenne P. Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy. Commun Biol 2025;. [PMID: 41315677]
- Jiang X, Xu P, Feng F, Grenci G, and Saw TB. Revealing Electromechanical Control of Tissue Homeostasis Using a Two-Layer Microfluidic Device. J Vis Exp 2025;(223). [PMID: 41052031]
- Cabillic M, Forriere H, Bettarel L, Butler C, Neuhaus A, Idrissi I, Sambrano-Lopez ME, Rossbroich J, Müller L, Ries J, Grenci G, Viasnoff V, Levet F, Sibarita J, and Galland R. In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy. Nat Commun 2025; 16(1):8362. [PMID: 40993142]
- Mu B, Rutkowski DM, Grenci G, Vavylonis D, and Zhang D. Ca2+-dependent vesicular and non-vesicular lipid transfer controls hypoosmotic plasma membrane expansion. BMC Biol 2025; 23(1):207. [PMID: 40629316]
- Ong HT, Karatas E, Poquillon T, Grenci G, Furlan A, Dilasser F, Mohamad Raffi SB, Blanc D, Drimaracci E, Mikec D, Galisot G, Johnson BA, Liu AZ, Thiel C, Ullrich O, , Racine V, and Beghin A. Digitalized organoids: integrated pipeline for high-speed 3D analysis of organoid structures using multilevel segmentation and cellular topology. Nat Methods 2025;. [PMID: 40369245]
- Arora A, Rizvi MS, Grenci G, Dilasser F, Fu C, Ganguly M, Vaishnavi S, Paramsivam K, Budnar S, Noordstra I, Yap AS, and Viasnoff V. Viscous dissipation in the rupture of cell-cell contacts. Nat Mater 2025;. [PMID: 40355570]
- Nakazawa N, Grenci G, Kameo Y, Takeda N, Sawada T, Kurisu J, Zhang Z, Toma K, Adachi T, Nonomura K, and Kengaku M. PIEZO1-dependent mode switch of neuronal migration in heterogeneous microenvironments in the developing brain. Cell Rep 2025; 44(3):115405. [PMID: 40053456]
- Suryana M, Produit T, Yang H, Birarda G, Shanmugar JV, Krivitsky L, Paterova A, and Grenci G. Infrared imaging with visible light in microfluidic devices: the water absorption barrier. Analyst 2024;. [PMID: 39692693]
Selected Publications
- Mona Suryana, Jegan V. Shanmugarajah, Sivakumar M. Maniam, Gianluca Grenci. Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light
- Mohammed Ashraf, Sree V. Sundararajan, Gianluca Grenci. Low-power, low-pressure reactive-ion etching process for silicon etching with vertical and smooth walls for mechanobiology application
Lab Members
Huang Yameng
Research Fellow, Low Group
Ang Jing Wen
Research Fellow, Class of August 2021, Low Group
Zuyang Aaron Fong
Research Assistant, Low Group
Nur Faezah Begum Binte Akbar Hussain
Research Assistant, Hanry Yu Group
The Chan lab is getting more culturally diverse!
The Chan lab welcomes research fellows Anna Jaeschke from Germany and Arikta Biswas from India! Anna will be applying her expertise on matrix biology and bioengineering to understand ovarian ageing, while Arikta will develop new imaging and biophysical approaches to study how mechanical stress impacts follicle morphogenesis and oocyte functions.
Nicole Loh Kit Ying
Alumni, Chan Group
Keeping a steady beat
AlumnNUS magazine interviews Prof Li and Prof Roger Foo from NUS Medicine about their ongoing collaboration that bridges basic science and clinical practice to understand the ageing heart.
Anna Jaeschke
Research Fellow, Chan Group
Probing follicle mechanical environment using light!
Our late work Mechanical mapping of mammalian follicle development using Brillouin microscopy is out in Communications Biology! Great collaboration with the Prevedel lab at EMBL Heidelberg.
Singapore Teaching and Academic Research Talent Award ceremony (Sep 27 2021)
The Chan lab is grateful to the START Inaugural Grant for supporting our research on understanding ovarian development, ageing and diseases in the coming years!
Celestine Ze Qing Ho
Research Assistant, Low Group
Cheam Mei Shan
Research Assistant, Toyama Group
Former Lab Members
Huang Yameng
Research Fellow, Low Group
Ang Jing Wen
Research Fellow, Class of August 2021, Low Group
Zuyang Aaron Fong
Research Assistant, Low Group
Nur Faezah Begum Binte Akbar Hussain
Research Assistant, Hanry Yu Group
The Chan lab is getting more culturally diverse!
The Chan lab welcomes research fellows Anna Jaeschke from Germany and Arikta Biswas from India! Anna will be applying her expertise on matrix biology and bioengineering to understand ovarian ageing, while Arikta will develop new imaging and biophysical approaches to study how mechanical stress impacts follicle morphogenesis and oocyte functions.
Nicole Loh Kit Ying
Alumni, Chan Group
Keeping a steady beat
AlumnNUS magazine interviews Prof Li and Prof Roger Foo from NUS Medicine about their ongoing collaboration that bridges basic science and clinical practice to understand the ageing heart.
Anna Jaeschke
Research Fellow, Chan Group
Probing follicle mechanical environment using light!
Our late work Mechanical mapping of mammalian follicle development using Brillouin microscopy is out in Communications Biology! Great collaboration with the Prevedel lab at EMBL Heidelberg.
Singapore Teaching and Academic Research Talent Award ceremony (Sep 27 2021)
The Chan lab is grateful to the START Inaugural Grant for supporting our research on understanding ovarian development, ageing and diseases in the coming years!
Celestine Ze Qing Ho
Research Assistant, Low Group
Cheam Mei Shan
Research Assistant, Toyama Group
