MBI Weekly Meeting Seminar

Time: 10.00am -11.00am
Date: Friday, 11 October 2019
Venue: Level 5 Seminar Room, T-Lab

Single cell analysis to dissect cellular heterogeneity in complex systems

By Dr. Cheow Lih Feng, Principal Investigator, National University of Singapore

Hosted by Prof. Kanchanawong

The human body is an amazing feat where trillions of cells of different cell types work together seamlessly to enable specific functions. Understanding the molecular mechanisms of these different cells is the key to understanding the basis of human health and conditions that leads to disease. To date, however, biological measurements are largely applied to bulk cells, which mask important cell-to-cell variations in tissues that give rise to diverse phenotypes. In this talk, I will present several approaches that we have developed to dissect various aspects of cellular heterogeneity. I will describe the Single Cell Restriction Analysis of Methylation (SCRAM) method that can be used to assay for DNA methylation states in single cells. I will also describe how it can be extended to the Single Cell analysis of Genotype, Expression and Methylation (sc-GEM) assay for multimodal analysis in single cells. Lastly I will present results from our latest DNA Analysis by Restriction Enzyme (DARE) assay that allows simultaneous genomic and epigenomic characterization of single cells. Applications for these assays will be presented.