MBI PhD Oral Defense
Time: 12noon
Date: Friday, 30 August 2019
Venue: Engineering Blk E3, Room E3-06-05
Supervisor: Prof Hanry Yu
Co-Supervisor: Prof Low Boon Chuan
Investigating Dynamics Of Bile Canaliculi Under Physiological Conditions And Obstructive Cholestasis
by Kapish GUPTA, Hanry Yu’s Group
Bile canaliculi (BC) expand and contract periodically to propel bile, however, the mechanism of BC contraction is not well understood. In the presentation I will discuss the role of intra canalicular pressure (ICP) in regulating BC dynamics via locally regulating biochemical signalling via Ca2+ sensing through Piezo-1 which in turn regulate pericanalicular actomyosin cortex (PAC) activity. I will also discuss about the homeostatic response to increased ICP wherein, PAC breaks locally leading to the formation of local membrane protrusion. Under physiological conditions, PAC can retract these protrusions to give rise to inward blebs (IB) like structures. However, beyond a certain threshold ICP (or in obstructive cholestasis), IB bud off the BC membrane as BC derived vesicles (BCV). BCV is a mechanism of bile regurgitation to manage the increased pressure in obstructive cholestasis.
**Please note the examination following the seminar is closed-door**
