What properties of the nucleus facilitate mechanotransduction? Similar to the concept of long distance force propagation along the cytoskeleton, the prestressed nuclear state, which counterbalances intracellular forces from the cytoskeleton, enables mechanotransduction [1][2]. Both the nuclear envelope and nuclear interior contribute to the mechanical [...]
How is nuclear “prestress” maintained? As an integral part of cellular behavior, cells are sensitive to matrix rigidity, local geometry and stress or strain applied by external factors [1]. In recent years, it has been established that an extensive network of protein assembly couples [...]
How is the nucleus coupled to the cytoskeleton? Cytoskeletal filaments bridge the nucleus to the plasma membrane, which in turn is anchored at sub-cellular sites to extracellular substrates via a plethora of proteins that form focal adhesions (FAs). FAs are points of cross-talk between [...]
What are nucleosomes? In order to fit DNA into the nucleus, it must be packaged into a highly compacted structure known as chromatin. In the first step of this process DNA is condensed into an 11 nm fiber that represents an approximate 6-fold level [...]
How does chromatin condensation keep the nucleus compact? Packaging of approximately a meter-long DNA duplex within the elastic nucleus determines its spatial 3D architecture and the genomic accessibility of tissue-specific transcriptional programs. Apart from several biochemical processes, there are various forces at play in [...]
How do chromosome territory dynamics affect gene redistribution? The spatial organization of chromatin within the 3-dimensional space of a chromosome territory enables the co-localization of co-transcribed genes and their transcriptional foci. Many gene positioning studies have shown that individual genes often loop out of [...]
What are transcription factories? The primary role of chromatin assembly is the compaction of an otherwise very long DNA molecule into discrete structures that fit into the nucleus. However, this compaction cannot be achieved at the expense of a cells ability to transcribe and [...]
How does chromatin remodeling impact gene transcription? While chromosome territory dynamics is believed to regulate gene expression through the redistribution of genes and the subsequent co-localization of these genes with transcription machinery, changes are also commonly made to the chromosome structure at a ‘local’ [...]
How is transcription regulated in stem cells? Pluripotent stem cells can potentially differentiate into any given cell type. They commonly exhibit mechanically softer nuclei [1], lack type-A lamins [2] and have a poorly defined cytoskeleton [3][4]. Embryonic stem cells are pluripotent in early organism [...]
How is DNA replicated? Cells must replicate their DNA before they can divide. This ensures that each daughter cell gets a copy of the genome, and therefore, successful inheritance of genetic traits. DNA replication is an essential process and the basic mechanism is conserved [...]
What is translation? Translation is a process that involves the synthesis of an amino acid chain from an mRNA blueprint. These polypeptide chains fold into functional proteins. Translation occurs outside the nucleus once nuclear processing of the pre-mRNA is complete and the mRNA molecules [...]
How do bacteria modulate the host cytoskeleton? Some bacterial pathogens gain entry and subvert immune detection by surviving intracellularly. Others remain extracellular, but manipulate host pathways from outside. A common strategy employed by both these pathogens is to modulate or subvert the host cellular [...]
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One of four Research Centres of Excellence at NUS, MBI is working to identify, measure and describe how the forces for motility and morphogenesis are expressed at the molecular, cellular and tissue level.