The East Asian Single-Molecule Biophysics Symposium 2025 (EASMB2025) was held from 24 to 26 November at the Ngee Ann Kongsi Auditorium in the Education Resource Centre, UTown, National University of Singapore. Chaired by Jie Yan, MBI and jointly organized with various institutions across South Korea, China, and Japan, the symposium showcased cutting-edge advances in single‑molecule biophysics across technology development, nucleic acids, mechanosensing, molecular motors, and biomolecular organization.

Across nine thematic sessions, the program featured 27 invited talks, nine selected short talks, and poster sessions that complemented the oral program, highlighting a diverse range of single‑molecule methods and applications. Participation was strong across East Asia, with international contributors—including those from Canada—underscoring the symposium’s broad regional reach and global relevance.

The scientific program reflected the field’s breadth and momentum, spanning next‑generation imaging, nucleic acids, molecular motors, mechanosensing, interfaces, and nanopores. Highlights included high‑precision live‑cell mapping; insights into force‑ and environment‑dependent control of recombination, replication, and chromatin; demonstrations of autonomous DNA motor–origami constructs and multi‑motor axonal transport; discoveries in mechanically tuned adhesion signaling; links between interfacial fields and condensates; and advances in multiplex single‑molecule sensing. Audience engagement was energetic throughout, with lively Q&A across sessions and posters.

Looking ahead, the next EASMB meeting will be hosted in Okinawa, where the community will continue to deepen regional collaboration and showcase new breakthroughs in single‑molecule biophysics. We look forward to your participation.