Wong Bin Sheng

Research Fellow, Mechanobiology Institute, National University of Singapore

mbiws@nus.edu.sg
Level 10 T-Lab
National University of Singapore
5A Engineering Drive 1
Singapore 117411

Wong Bin Sheng

Research Fellow

Principal Investigator

Rong Li

Research Areas:

Cell migration, Cell cycle and mitosis, Cell mechanics and dynamics

Qualifications:

  • Ph.D. (Chemical and Biomolecular Engineering), Johns Hopkins University (JHU)
  • B.Sc. (Pharmacy), National University of Singapore (NUS)

Biography:

Bin Sheng received his B.Sc. in Pharmacy with First Class Honours from NUS (2009-2013), and graduated with a Ph.D. in Chemical and Biomolecular Engineering from JHU under the guidance of Professor Konstantinos Konstantopoulos (2013-2019). By employing an interdisciplinary approach that combines bioengineering and microfabrication, molecular and cell biology, biochemistry, quantitative image analysis and in vivo preclinical models, his doctoral research focused on understanding the biochemical and physicomechanical mechanisms of cell migration and how these basic science discoveries can be translated into potential clinical tools against cancer metastasis.

He is currently a postdoctoral research fellow for Professor Rong Li at the Mechanobiology Institute, NUS (2020-now), where he is extending his research to the area of mechanobiology and cellular dynamics. Specifically, he is working on understanding how mammalian cells behave and adapt to chromosomal imbalance and chronic osmotic stress.

Technical Skills:

  • Cell Biology: Bacterial and mammalian cell culture, transient and stable transfection, lentivirus production and transduction, CRISPR, cell line generation, cell sorting
  • Molecular Biology: Molecular cloning, DNA/RNA/protein isolation, plasmid generation, primer design, QPCR, western blotting, zymography, cell viability assays
  • Biochemistry: ELISA, immunoprecipitation, in vitro pulldown assays, recombinant protein design, production and purification, mass spectrometry
  • Microscopy: Time-lapse live cell imaging, epifluorescence microscopy, confocal microscopy, TIRF microscopy, immunofluorescence staining, FRAP/FLIP, FRET/FLIP
  • Screening: RNA sequencing, CRISPR knockout genomic screen, chemogenomic screen with drug inhibitors library
  • Microfabrication: Microfluidic device design and fabrication, photolithography, replica molding, microcontact printing, polyacrylamide hydrogel preparation, cell seeding and isolation from microfluidic devices
  • In vivo work: Organoid harvesting and isolation, subcutaneous injection, orthotopic implantation, hemispleen hepatic metastasis model, microsurgery, immunohistochemistry

Selected Publications

  1. Tuntithavornwat, S., Shea, D. J., Wong, B. S., Guardia, T., Lee, S. J., Yankaskas, C. L., … & Konstantopoulos, K. (2022). Giant obscurin regulates migration and metastasis via RhoA-dependent cytoskeletal remodeling in pancreatic cancer. Cancer letters, 526, 155-167. https://doi.org/10.1016/j.canlet.2021.11.016
  2. Wong, B. S., Shah, S. R., Yankaskas, C. L., Bajpai, V. K., Wu, P. H., Chin, D., … & Konstantopoulos, K. (2021). A microfluidic cell-migration assay for the prediction of progression-free survival and recurrence time of patients with glioblastoma. Nature Biomedical Engineering, 5(1), 26-40. https://doi.org/10.1038/s41551-020-00621-9
  3. Guerrero, P. E., Miró, L., Wong, B. S., Massaguer, A., Martínez-Bosch, N., Llorens, R. D., … & Peracaula, R. (2020). Knockdown of α2, 3-sialyltransferases impairs pancreatic cancer cell migration, invasion and e-selectin-dependent adhesion. International Journal of Molecular Sciences, 21(17), 6239. https://doi.org/10.3390/ijms21176239
  4. Wong, B. S., Shea, D. J., Mistriotis, P., Tuntithavornwat, S., Law, R. A., Bieber, J. M., … & Konstantopoulos, K. (2019). A direct podocalyxin–dynamin-2 interaction regulates cytoskeletal dynamics to promote migration and metastasis in pancreatic cancer cells. Cancer Research, 79(11), 2878-2891. https://doi.org/10.1158/0008-5472.can-18-3369
  5. Wong, B. S., Mistriotis, P., & Konstantopoulos, K. (2018). Exposing cell-itary confinement: Understanding the mechanisms of confined single cell migration. Biomechanics in Oncology, 139-157. https://doi.org/10.1007/978-3-319-95294-9_8