Super-Resolution Microscopy of EnvZ, OmpR, H-NS, and Chromosomal Imaging

We have constructed photoactivatable fusions to EnvZ, OmpR and H-NS and are now imaging these proteins in various strain backgrounds and in response to numerous environmental stresses, including high osmolality and acid pH. We have also been examining OmpR/DNA interactions using PALM.

STORM image of pSL5 (EnvZ-PAmCherry) in E.coli MG1655. Cells were grown in A medium with 1 mM IPTG induction for 8 h. Scale bar is 2 μm.

Researchers: Hong Fang Zheng, Stuti Desai
Lab: Linda J Kenney Lab