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PRODID:-//Mechanobiology Institute, National University of Singapore - ECPv6.15.12.2//NONSGML v1.0//EN
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METHOD:PUBLISH
X-ORIGINAL-URL:https://www.mbi.nus.edu.sg
X-WR-CALDESC:Events for Mechanobiology Institute, National University of Singapore
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BEGIN:VTIMEZONE
TZID:Asia/Singapore
BEGIN:STANDARD
TZOFFSETFROM:+0800
TZOFFSETTO:+0800
TZNAME:+08
DTSTART:20180101T000000
END:STANDARD
END:VTIMEZONE
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190301T093000
DTEND;TZID=Asia/Singapore:20190301T103000
DTSTAMP:20260419T225927
CREATED:20190226T030715Z
LAST-MODIFIED:20190226T030715Z
UID:25973-1551432600-1551436200@www.mbi.nus.edu.sg
SUMMARY:Re-engineering Focal Adhesions by Tony Kanchanawong
DESCRIPTION:MBI Weekly Meeting Seminar\nTime: 9.30am-10.30am\nDate: Friday\, 1 March 2019\nVenue: Level 5 Seminar Room\, T-Lab \nRe-engineering Focal Adhesions\nby Prof. Pakorn Tony Kanchanawong\, Associate Professor\, Mechanobiology Institute\, National University of Singapore.
URL:https://www.mbi.nus.edu.sg/event/re-engineering-focal-adhesions-by-tony-kanchanawong/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Weekly Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190304T160000
DTEND;TZID=Asia/Singapore:20190304T170000
DTSTAMP:20260419T225927
CREATED:20190215T022732Z
LAST-MODIFIED:20190215T022732Z
UID:25910-1551715200-1551718800@www.mbi.nus.edu.sg
SUMMARY:Identification Of Calcium Oxalate Monohydrate Crystal Receptors And Molecular Mechanisms Underlying Microvillar Injury In Response To Calcium Oxalate Monohydrate Crystal Exposure Of Renal Tubular Epithelial Cells by Kedsarin Fong-ngern
DESCRIPTION:We are pleased to inform you on the seminar of Kedsarin Fong-ngern (post doc candidate)\, hosted by A/Prof Tony Kanchanawong “Identification Of Calcium Oxalate Monohydrate Crystal Receptors And Molecular Mechanisms Underlying Microvillar Injury In Response To Calcium Oxalate Monohydrate Crystal Exposure Of Renal Tubular Epithelial Cells” on 4 March (Mon) @4pm at T-Lab Level 5 Seminar Rooms.
URL:https://www.mbi.nus.edu.sg/event/identification-of-calcium-oxalate-monohydrate-crystal-receptors-and-molecular-mechanisms-underlying-microvillar-injury-in-response-to-calcium-oxalate-monohydrate-crystal-exposure-of-renal-tubular-epit/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190305T093000
DTEND;TZID=Asia/Singapore:20190305T103000
DTSTAMP:20260419T225927
CREATED:20190306T071017Z
LAST-MODIFIED:20190306T071017Z
UID:26015-1551778200-1551781800@www.mbi.nus.edu.sg
SUMMARY:MBI SPSS: Characterization of multidrug resistant\, invasive non-typhoidal Salmonella (iNTS) VNS20081by Gao Yunfeng
DESCRIPTION:MBI Student and Postdoc Seminar Series (SPSS)\nTime: 9.30am-10.30am\nDate: Tuesday\, 5 March 2019\nVenue: Level 5 Seminar Room\, T-Lab \nCharacterization of multidrug resistant\, invasive non-typhoidal Salmonella (iNTS) VNS20081\nby Gao Yunfeng\, Research Associate\, Kenney Group \n  \n* Please note this event is open to MBI researchers and invited guests only. Thank you *
URL:https://www.mbi.nus.edu.sg/event/mbi-spss-characterization-of-multidrug-resistant-invasive-non-typhoidal-salmonella-ints-vns20081by-gao-yunfeng/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Student & Postdoc Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190306T150000
DTEND;TZID=Asia/Singapore:20190306T160000
DTSTAMP:20260419T225927
CREATED:20190306T064039Z
LAST-MODIFIED:20190306T064039Z
UID:26000-1551884400-1551888000@www.mbi.nus.edu.sg
SUMMARY:MBI PhD Oral Defense: T. brucei Spef1/CLAMP is required for anchorage of cytoskeletal organelles to the basal bodiesby Dong Xiaoduo
DESCRIPTION:MBI PhD Oral Defense\nTime: 3pm\nDate: 6 March 2019\, Wednesday\nVenue: NUS\, E3-06-04 \nSupervisor(s): Dr Low Boon Chuan (main sup)\, Dr Cynthia He (co-sup) \nT. brucei Spef1/CLAMP is required for anchorage of cytoskeletal organelles to the basal bodies\nby DONG Xiaoduo\,  Low Boon Chuan Group \nSperm flagellar protein 1\, also known as Spef1/CLAMP\, was first discovered in mouse sperm flagellum and has been implicated in spermatogenesis and central pair assembly. It binds and bundles microtubules (MTs)\, and facilitates embryonic intercalation with its MT functions in Xenopus. Additionally\, it has been shown to play a role in planar cell polarity\, which underlies global polarity at tissue level and is essential for a large variety of developmental processes and collective cell behaviors. \nTrypanosoma brucei (T. brucei)\, the causative agent of trypanosomiasis\, is an excellent model for organelle biology studies for its simple cellular organization with a series of single-copied organelles\, which replicate and divide following a strict temporal and spatial order.Previously\, T. brucei  Spef1 ortholog (TbSpef1) has been shown to be associated with a specialized microtubule quartet (MTQ)\, which originates from a region between the pro- and mature basal bodies. Knockdown of TbSpef1 by RNA interference (RNAi) results in defects in MTQ assembly and duplication/segregation of several single-copied cytoskeletal organelles\, suggesting a role of TbSpef1 in co-ordinated organelle biogenesis/division during the T. bruceicell cycle. \nIn this study\, the microtubule binding and bundling effects of TbSpef1 were demonstrated by in vitro assays. Using BioID\, a protein-protein interaction screening method\, 37 putative interacting partners of TbSpef1 have been identified with 8 further characterized. All 8 proteins were in close association to TbSpef1\, validating the proximity-based method.One candidate was found to be localized between the pro- and mature basal bodies. RNAi of this protein did not affect duplication of the basal bodies nor any other organelles\, but severed the connection between the MTQ and the basal bodies. This led to changes in the positioning of several cytoskeletal organelles relative to the basal bodies\, and morphological changes in membrane-bound organelles including the flagellar pocket (FP). This study provided the first molecular identification of the connection between the MTQ and the basal bodies in T. brucei. The results also revealed a conserved cellular function of Spef1 in polarized cell development mediated by MT anchorage\, possibly via its MT binding and bundling activities. \n  \n**Please note the examination following the seminar is closed-door**
URL:https://www.mbi.nus.edu.sg/event/mbi-phd-oral-defense-t-brucei-spef1-clamp-is-required-for-anchorage-of-cytoskeletal-organelles-to-the-basal-bodiesby-dong-xiaoduo/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:PhD Oral Defense
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190306T150000
DTEND;TZID=Asia/Singapore:20190306T180000
DTSTAMP:20260419T225927
CREATED:20190306T064405Z
LAST-MODIFIED:20190306T064405Z
UID:26002-1551884400-1551895200@www.mbi.nus.edu.sg
SUMMARY:MBI Outreach: MBI welcomes students & teachers from Hokkaido University
DESCRIPTION:MBI Outreach Event\nThere will be a MBI Outreach Visit by Hokkaido University on 6th March\, Wednesday: \nDate: 6th March 2019\, Wednesday\nTime: 3.00 – 6.00PM\nPax: 26 students and 2 teachers \nLab demonstrations: estimated to be from 4.30 – 5.30PM \n\nWidefield Fluorescence Microscope (L9 Microscopy Room 8) – venue has been changed to L10 Room 1 (Delta Vision)\nMicrofabrication (L9 lab benches)\nSuperresolution (L9 NSTORM system room 7)\n\nTherefore\, we would like to request your kind cooperation to plan your experiments with this in mind\, so as to ensure the smooth progress of the lab demos from 4.30-5.30PM. \nThank you.
URL:https://www.mbi.nus.edu.sg/event/mbi-outreach-mbi-welcomes-students-teachers-from-hokkaido-university/
LOCATION:National University of Singapore
CATEGORIES:Outreach Event
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190307T160000
DTEND;TZID=Asia/Singapore:20190307T170000
DTSTAMP:20260419T225927
CREATED:20190306T063736Z
LAST-MODIFIED:20190306T063736Z
UID:25997-1551974400-1551978000@www.mbi.nus.edu.sg
SUMMARY:The Segmental Pattern Of The Zebrafish Axial Skeleton Arises From The Notochord Sheath by Rachna Narayanan
DESCRIPTION:MBI Seminar\nWe are pleased to inform you on the seminar of Rachna Narayanan (post doc candidate)\, hosted by Dr Timothy Saunders “The Segmental Pattern Of The Zebrafish Axial Skeleton Arises From The Notochord Sheath” on 7 Mar (Thurs) @4pm at T-Lab Level 5 Seminar Rooms.
URL:https://www.mbi.nus.edu.sg/event/the-segmental-pattern-of-the-zebrafish-axial-skeleton-arises-from-the-notochord-sheath-by-rachna-narayanan/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190308T093000
DTEND;TZID=Asia/Singapore:20190308T103000
DTSTAMP:20260419T225927
CREATED:20190306T065851Z
LAST-MODIFIED:20190306T065851Z
UID:26006-1552037400-1552041000@www.mbi.nus.edu.sg
SUMMARY:MBIw: Imaging Synapses: From Molecule to Function by Jun Nishiyama\, Duke-NUS Medical School
DESCRIPTION:MBI Weekly Meeting Seminar\nTime: 9.30am-10.30am\nDate: Friday\, 8 March 2019\nVenue: Level 5 Seminar Room\, T-Lab \nImaging Synapses: From Molecule to Function\nby Prof. Jun Nishiyama\, Assistant Professor\, Duke-NUS Medical School \nOur brain functions depend on connections between billions of neurons. These connections or synapses are believed to be disrupted in many neuropsychiatric disorders such as autism\, schizophrenia\, and Alzheimer’s disease. However\, molecular mechanisms underlying synaptic function and dysfunction are largely unknown. In this seminar\, I will introduce our studies to probe endogenous proteins and image signal transduction in single synapses using cutting-edge genome editing and various optical techniques. I will discuss how these tools can help to understand molecular regulation of synapses. \nReferences\n1) Nishiyama\, J.*\, Mikuni\, T*\,†.\, Yasuda R†. Virus-Mediated Genome Editing via Homology-Directed Repair in Mitotic and Postmitotic Cells in Mammalian Brain. Neuron\, 96\, 755-768 (2017). \n2) Mikuni\, T.*\, Nishiyama\, J.*\,†\, Sun Y.\, Kamasawa N.\, Yasuda\, R.† High-Throughput\, High-Resolution Mapping of Protein Localization in Mammalian Brain by In Vivo Genome Editing. Cell\, 165 1803-17 (2016). \n3) Nishiyama\, J. and Yasuda\, R. Biochemical Computation for Spine Structural Plasticity. Neuron\, 87 63-75 (2015).
URL:https://www.mbi.nus.edu.sg/event/mbiw-imaging-synapses-from-molecule-to-function-by-jun-nishiyama-duke-nus-medical-school/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Weekly Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190312T093000
DTEND;TZID=Asia/Singapore:20190312T103000
DTSTAMP:20260419T225927
CREATED:20190311T031516Z
LAST-MODIFIED:20190311T031516Z
UID:26052-1552383000-1552386600@www.mbi.nus.edu.sg
SUMMARY:MBI SPSS: Approach for understanding Salmonella Typhi infection and evolution by Yuki Yamanaka
DESCRIPTION:MBI Student and Postdoc Seminar Series (SPSS)\nTime: 9.30am-10.30am\nDate: Tuesday\, 12 March 2019\nVenue: Level 5 Seminar Room\, T-Lab \nApproach for understanding Salmonella Typhi infection and evolution\nby Yuki Yamanaka\, Research Fellow\, Kenney Group \n  \n* Please note this event is open to MBI researchers and invited guests only. Thank you *
URL:https://www.mbi.nus.edu.sg/event/mbi-spss-approach-for-understanding-salmonella-typhi-infection-and-evolution-by-yuki-yamanaka/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Student & Postdoc Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190313T120000
DTEND;TZID=Asia/Singapore:20190313T130000
DTSTAMP:20260419T225927
CREATED:20190308T060152Z
LAST-MODIFIED:20190308T060300Z
UID:26025-1552478400-1552482000@www.mbi.nus.edu.sg
SUMMARY:Bacterial Type VI secretion system: from discovery to dynamics and mode of action by Marek Basler
DESCRIPTION:We are pleased to inform you on the seminar by Prof. Marek Basler\, hosted by Prof. Linda Kenney “Bacterial Type VI secretion system: from discovery to dynamics and mode of action” on 13 Mar (Wed) @ 12pm at T-Lab Level 5 Seminar Rooms. Thank you. \nLearn more about Prof. Basler.
URL:https://www.mbi.nus.edu.sg/event/bacterial-type-vi-secretion-system-from-discovery-to-dynamics-and-mode-of-action-by-marek-basler/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190313T130000
DTEND;TZID=Asia/Singapore:20190313T140000
DTSTAMP:20260419T225927
CREATED:20190307T044014Z
LAST-MODIFIED:20190307T044014Z
UID:26020-1552482000-1552485600@www.mbi.nus.edu.sg
SUMMARY:MBI PhD Qualifying Exam: Mechanotransduction pathways regulating the geometric reprogramming of fibroblasts by Yuan Luezhen
DESCRIPTION:MBI PhD Qualifying Exam\nTime: 1pm\nDate: 13 March 2019\, Wednesday\nVenue: MBI\, Level 5 Meeting Room. \nSupervisor: Dr GV Shivashankar \nMechanotransduction pathways regulating the geometric reprogramming of fibroblasts\nBy Yuan Luezhen\, Shivashankar Lab \nOur laboratory recently discovered that lateral confinement growth induces reprogramming of fibroblasts to embryonic stem cell-like states without exogenous expression of reprogramming factors. This highlights the role of cell geometry in regulating cell state switching. However\, the detailed mechanobiology\, especially how cell geometry could precisely regulate gene expression through altering the activity of transcription factors and controlling epigenetics states is largely unknown. The goal of my Ph.D. project is to elucidate the mechanism of lateral confinement growth induced reprogramming process.  In this presentation\, I will show the rationale of this project along with the preliminary results demonstrating the role of Lef1 and β-catenin in this reprogramming process. My proposed research will have important implications in increasing the efficiency of reprogramming and also contribute towards understanding the role of cell geometry control in cell state switching in disease progression. \n  \n**Please note the examination following the seminar is closed-door** \n 
URL:https://www.mbi.nus.edu.sg/event/mbi-phd-qualifying-exam-mechanotransduction-pathways-regulating-the-geometric-reprogramming-of-fibroblasts-by-yuan-luezhen/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:PhD Oral Defense
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190313T160000
DTEND;TZID=Asia/Singapore:20190313T170000
DTSTAMP:20260419T225927
CREATED:20190306T070204Z
LAST-MODIFIED:20190306T070204Z
UID:26008-1552492800-1552496400@www.mbi.nus.edu.sg
SUMMARY:Control And Self-Organization Of Tissue Morphogenesis by Thomas Lecuit
DESCRIPTION:We are pleased to inform you on the seminar of Thomas Lecuit\, hosted by A/Prof Virgile Viasnoff “Control And Self-Organization Of Tissue Morphogenesis” on 13 Mar (Wed) @4pm at T-Lab Level 5 Seminar Rooms.
URL:https://www.mbi.nus.edu.sg/event/control-and-self-organization-of-tissue-morphogenesis-by-thomas-lecuit/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190314T113000
DTEND;TZID=Asia/Singapore:20190314T123000
DTSTAMP:20260419T225927
CREATED:20190226T035030Z
LAST-MODIFIED:20190226T035030Z
UID:25990-1552563000-1552566600@www.mbi.nus.edu.sg
SUMMARY:The Emergence of Tissue Mechanics from Confinement of Non-linear Biopolymer Networks by Densely Packed Cells by Vivek Shenoy
DESCRIPTION:We are pleased to inform you on the seminar of Prof. Vivek Shenoy\, hosted by Prof. GV Shivashankar\, “The Emergence of Tissue Mechanics from Confinement of Non-linear Biopolymer Networks by Densely Packed Cells ” on 14 March (Thurs) @11.30am at T-Lab Level 5 Seminar Rooms.
URL:https://www.mbi.nus.edu.sg/event/the-emergence-of-tissue-mechanics-from-confinement-of-non-linear-biopolymer-networks-by-densely-packed-cells-by-vivek-shenoy/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190314T150000
DTEND;TZID=Asia/Singapore:20190314T160000
DTSTAMP:20260419T225927
CREATED:20190306T070538Z
LAST-MODIFIED:20190306T070713Z
UID:26011-1552575600-1552579200@www.mbi.nus.edu.sg
SUMMARY:A Journey\, from 3D Perception of Motion\, to Machine Vision\, to Machine Learning Applied to Nonlinear Dynamics\, to Allometry\, to Bioinformatics by Lim Teck Por
DESCRIPTION:We are pleased to inform you on the seminar of Lim Teck Por (post doc candidate)\, hosted by Dr Timothy Saunders “A Journey\, from 3D Perception of Motion\, to Machine Vision\, to Machine Learning Applied to Nonlinear Dynamics\, to Allometry\, to Bioinformatics” on 14 Mar (Thurs) @3pm at T-Lab Level 5 Seminar Rooms.
URL:https://www.mbi.nus.edu.sg/event/a-journey-from-3d-perception-of-motion-to-machine-vision-to-machine-learning-applied-to-nonlinear-dynamics-to-allometry-to-bioinformatics-by-lim-teck-por/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190315T093000
DTEND;TZID=Asia/Singapore:20190315T103000
DTSTAMP:20260419T225927
CREATED:20190312T014518Z
LAST-MODIFIED:20190312T014518Z
UID:26056-1552642200-1552645800@www.mbi.nus.edu.sg
SUMMARY:MBIw: The Last Seminar: A retrospective on my past 5 years at MBI and in Singapore\, and a synopsis of my 40 years of academic research: Three items of friendly advice for young scholars by Marius Sudol
DESCRIPTION:MBI Weekly Meeting Seminar\nTime: 9.30am-10.30am\nDate: Friday\, 15 March 2019\nVenue: Level 5 Seminar Room\, T-Lab \nThe Last Seminar: A retrospective on my past 5 years at MBI and in Singapore\, and a synopsis of my 40 years of academic research: Three items of friendly advice for young scholars\nby Prof. Marius Sudol\, Co-PI at Mechanobiology Institute and Associate Professor at Department of Physiology\, National University of Singapore.
URL:https://www.mbi.nus.edu.sg/event/mbiw-the-last-seminar-a-retrospective-on-my-past-5-years-at-mbi-and-in-singapore-and-a-synopsis-of-my-40-years-of-academic-research-three-items-of-friendly-advice-for-young-scholars-by-marius-sudo/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Weekly Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190315T143000
DTEND;TZID=Asia/Singapore:20190315T153000
DTSTAMP:20260419T225927
CREATED:20190311T060741Z
LAST-MODIFIED:20190311T060741Z
UID:26054-1552660200-1552663800@www.mbi.nus.edu.sg
SUMMARY:MBI PhD Exam: The Role of SsrB in Salmonella Typhimurium Lifestyle by Dasvit Shetty
DESCRIPTION:MBI PhD Qualifying Exam\nTime: 2.30pm\nDate: 15 March 2019\, Friday\nVenue: MBI\, Level 5 Meeting Room. \nSupervisor: Prof. Linda J. Kenney \nThe Role of SsrB in Salmonella Typhimurium Lifestyle\nby Dasvit SHETTY\, Kenney Group \nSsrB is a response regulator that controls the induction of SPI-2 genes during the intracellular phase of Salmonella Typhimurium infection. SsrB is a NarL/FixJ family response regulator and is phosphorylated by its associated sensor kinase SsrA in order to activate SPI-2 genes. An unphosphorylated SsrB mutant\, D56A\, was discovered to regulate the master biofilm regulator csgD\, thus functioning as a lifestyle switch between the infectious and carrier states of the pathogen. This discovery led to an RNA-seq analysis to investigate more genes that might be under SsrB control during the non-virulent lifestyle. Analysis of the RNA-seq data of wild-type and DssrB strains in biofilm-inducing conditions showed down-regulation of many stress response genes in the DssrB strain\, most notably dksA\, dsrA and hfq genes. These genes are known regulators of the ancestral stress sigma factor RpoS; and were unaffected in the D56A ssrB strain. This study aims to explore the role of SsrB in its non-canonical regulation of RpoS through DksA\, DsrA and Hfq. \nAlthough D56A SsrB has been shown to bind to DNA using AFM\, it is unknown whether its binding affinity is comparable to wild-type phosphorylated SsrB (SsrB~P). Measuring the dissociation constants of D56A SsrB and SsrB~P to its known cognate promoters using a single molecule unzipping assay will assist in understanding the role of phosphorylation in DNA affinity and promoter-specificity of SsrB. Another unique characteristic of SsrB among NarL/FixJ regulators is its increased DNA binding at low pH. Residues in SsrB that influence this property and the molecular mechanism are not known. Hence\, this study aims to use NMR and a single molecule unzipping assay to elucidate the basis of the increased DNA affinity of SsrB at low pH. \n  \n**Please note the examination following the seminar is closed-door**
URL:https://www.mbi.nus.edu.sg/event/mbi-phd-exam-the-role-of-ssrb-in-salmonella-typhimurium-lifestyle-by-dasvit-shetty/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:PhD Oral Defense
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190319T093000
DTEND;TZID=Asia/Singapore:20190319T103000
DTSTAMP:20260419T225927
CREATED:20190314T094502Z
LAST-MODIFIED:20190314T094543Z
UID:26155-1552987800-1552991400@www.mbi.nus.edu.sg
SUMMARY:MBI SPSS: Interaction between Salmonella and tumor aggregates on chip by Xu Lisheng
DESCRIPTION:MBI Student and Postdoc Seminar Series (SPSS)\nTime: 9.30am-10.30am\nDate: Tuesday\, 19 March 2019\nVenue: Level 5 Seminar Room\, T-Lab \nInteraction between Salmonella and tumor aggregates on chip\nby Xu Lisheng\, PhD Student \, Kenney Group \n  \n* Please note this event is open to MBI researchers and invited guests only. Thank you *
URL:https://www.mbi.nus.edu.sg/event/mbi-spss-interaction-between-salmonella-and-tumor-aggregates-on-chip-by-xu-lisheng/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Student & Postdoc Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190320T150000
DTEND;TZID=Asia/Singapore:20190320T160000
DTSTAMP:20260419T225927
CREATED:20190312T040648Z
LAST-MODIFIED:20190312T040648Z
UID:26059-1553094000-1553097600@www.mbi.nus.edu.sg
SUMMARY:Seeing how life starts: Imaging the dynamics that form the mammalian embryo by Nicolas Plachta
DESCRIPTION:MBI Seminar\nWe are pleased to inform you on the seminar of MBI Seminar: Dr. Nicolas Plachta\, hosted by Prof. Yusuke Toyama “Seeing how life starts: Imaging the dynamics that form the mammalian embryo“ on 20 Mar (Wed) @3pm at T-Lab Level 5 Seminar Rooms. Thank you.
URL:https://www.mbi.nus.edu.sg/event/seeing-how-life-starts-imaging-the-dynamics-that-form-the-mammalian-embryo-by-nicolas-plachta/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190320T160000
DTEND;TZID=Asia/Singapore:20190320T170000
DTSTAMP:20260419T225927
CREATED:20190313T044144Z
LAST-MODIFIED:20190313T044144Z
UID:26096-1553097600-1553101200@www.mbi.nus.edu.sg
SUMMARY:Epithelial Cell Size Dynamics And Inter-Tissue Cooperation During Thorax Closure In Drosophila by Thamarailingam A
DESCRIPTION:We are pleased to inform you on the seminar of MBI Seminar: Thamarailingam A. (post doc candidate)\, hosted by Dr Timothy Saunders “Epithelial Cell Size Dynamics And Inter-Tissue Cooperation During Thorax Closure In Drosophila” on 20 Mar (Wed) @4pm at T-Lab Level 5 Seminar Rooms. Thank you.
URL:https://www.mbi.nus.edu.sg/event/epithelial-cell-size-dynamics-and-inter-tissue-cooperation-during-thorax-closure-in-drosophila-by-thamarailingam-a/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190322T093000
DTEND;TZID=Asia/Singapore:20190322T103000
DTSTAMP:20260419T225927
CREATED:20190314T025949Z
LAST-MODIFIED:20190320T013738Z
UID:26141-1553247000-1553250600@www.mbi.nus.edu.sg
SUMMARY:CANCELLED: Cell-Extracellular Matrix Adhesion: Molecular Basis\, Signaling And Diseases by Wu Chuanyue
DESCRIPTION:MBI Seminar\nWe are pleased to inform you on the seminar of Prof. Wu Chuanyue\, hosted by Prof. Yan Jie\, “Cell-Extracellular Matrix Adhesion: Molecular Basis\, Signaling And Diseases” on 22 March (Frid) @9.30am at T-Lab Level 5 Seminar Rooms. Thank you.
URL:https://www.mbi.nus.edu.sg/event/cell-extracellular-matrix-adhesion-molecular-basis-signaling-and-diseases-by-wu-chuanyue/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190322T140000
DTEND;TZID=Asia/Singapore:20190322T150000
DTSTAMP:20260419T225927
CREATED:20190313T043802Z
LAST-MODIFIED:20190313T043802Z
UID:26093-1553263200-1553266800@www.mbi.nus.edu.sg
SUMMARY:RHO-1 GEF RHGF-1 promotes actomyosin contractility in C. elegans spermatheca by Kriti Sethi
DESCRIPTION:We are pleased to inform you on the seminar of MBI Seminar: Kriti Sethi (post doc candidate)\, hosted by Dr Timothy Saunders “RHO-1 GEF RHGF-1 promotes actomyosin contractility in C. elegans spermatheca” on 22 Mar (Fri) @2pmat T-Lab Level 5 Seminar Rooms. Thank you.
URL:https://www.mbi.nus.edu.sg/event/rho-1-gef-rhgf-1-promotes-actomyosin-contractility-in-c-elegans-spermatheca-by-kriti-sethi/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20190326
DTEND;VALUE=DATE:20190329
DTSTAMP:20260419T225927
CREATED:20190211T063827Z
LAST-MODIFIED:20190212T035014Z
UID:25836-1553558400-1553817599@www.mbi.nus.edu.sg
SUMMARY:Workshop on quantitative single molecule imaging with world experts
DESCRIPTION:Workshop on quantitative single molecule imaging with world experts\nMarch 26th to 28th\nMechanobiology Institute\nOrganizers\nV. Viasnoff\, J.B. Sibarita\, T. Wohland\, G.Wright\nTopics\n\nSingle molecule-based super-resolution microscopy\nSingle molecule localization and image reconstruction cluster analysis\nFluorescence correlation spectrocopy\n\nProgram\n(Mornings: theory\, Afternoons: hands-on (capped to 20 participants)) \nTuesday March 26th\nMorning \n\nFluorescence microscopy and single particle techniques (Eric Hosy\, CNRS)\nFluorescence correlation spectroscopy (Thorsten Wohland\, DBS)\n\nAfternoon \n\nHands on parallel sessions: SM imaging/data analysis. Venue: MBI\n\nWednesday March 27th\nMorning \n\nSingle molecule data quantification and image reconstruction (Jean-Bapiste Sibarita\, CNRS)\nExamples of super-resolution imaging capabilities within Singascope (Graham Wright\, IMB)\n\nAfternoon \n\nHands on parallel sessions: SM imaging/data analysis. Venue: MBI\n\nThursday March 28th\nMorning \n\nAdvanced single molecule imaging (Jean-Bapiste Sibarita\, CNRS)\nTBA (Felix Margadant\, NUS)\nTBA (Tony Kanchanawong\, NUS)\nProbing transcription factors dynamics by FCS (Nicolas Plachta\, IMCB)\n\nAfternoon \n\nHands on FCS technics (single point\, 2D). Venue: DBS\n\n  \nRegistration is FREE but MANDATORY. Register here or send an email to dbsvvnr@nus.edu.sg
URL:https://www.mbi.nus.edu.sg/event/workshop-on-quantitative-single-molecule-imaging-with-world-experts/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:Workshop
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190328T100000
DTEND;TZID=Asia/Singapore:20190328T110000
DTSTAMP:20260419T225927
CREATED:20190325T100619Z
LAST-MODIFIED:20190325T100619Z
UID:26214-1553767200-1553770800@www.mbi.nus.edu.sg
SUMMARY:PhD Qualifying Exam: Harnessing the proliferative activity of YAP through optogenetics for regenerative medicine by Toh Jia Ying Pearlyn
DESCRIPTION:MBI PhD Qualifying Exam\nTime: 10am\nDate: 28 March 2019\, Thursday\nVenue: NUS\, E3-06-13 Tutorial Room \nSupervisor: Prof Michael Sheetz \nHarnessing the proliferative activity of YAP through optogenetics for regenerative medicine\nBy: Toh Jia Ying Pearlyn\, Sheetz Lab \nThe Hippo-YAP pathway is crucial in organ size control during development and regeneration\, and during pathological processes such as tumorigenesis. One downstream transcriptional co-activator Yes-associated protein (YAP) is a key effector of the pathway\, where its activity and localization regulate cell proliferation\, apoptosis\, and carcinogenesis. It has been shown that YAP can promote regeneration in mice liver\, but therapeutic activation of YAP can have negative side effects as prolonged activation causes cancer development in mice. Therefore\, my project aims to develop a tightly spatial and temporal controlled activation system of YAP using optogenetics to harness its proliferative activity in a safe way. A transgenic zebrafish model that harbors the optogenetic YAP will be generated to study fin regeneration during the larval stage. These developments will contribute to understanding and paving the way for YAP in regenerative medicine. \n  \n**Please note the examination following the seminar is closed-door**
URL:https://www.mbi.nus.edu.sg/event/phd-qualifying-exam-harnessing-the-proliferative-activity-of-yap-through-optogenetics-for-regenerative-medicine-by-toh-jia-ying-pearlyn/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:PhD Oral Defense
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190329T093000
DTEND;TZID=Asia/Singapore:20190329T103000
DTSTAMP:20260419T225927
CREATED:20190326T074933Z
LAST-MODIFIED:20190326T074933Z
UID:26226-1553851800-1553855400@www.mbi.nus.edu.sg
SUMMARY:MBIw: Spatially restricted proteomics of the polarity proteins Par3 and Pals1 reveals sub-compartmentalization of the epithelial apical-lateral membrane border by Alexander Ludwig
DESCRIPTION:MBI Weekly Meeting Seminar\nTime: 9.30am-10.30am\nDate: Friday\, 29 March 2019\nVenue: Level 5 Seminar Room\, T-Lab \nSpatially restricted proteomics of the polarity proteins Par3 and Pals1 reveals sub-compartmentalization of the epithelial apical-lateral membrane border\nBy Alexander Ludwig\, Assistant Professor\, School of Biological Sciences and Nanyang Institute of Structural Biology\, Nanyang Technological University\, Singapore \nIn mammalian epithelial cells the Par\, Crumbs and Scribble polarity modules occupy distinct apical and lateral membrane compartments that overlap at the level of tight junctions (TJ). However\, the precise sub-junctional organization of the polarity proteins remains unclear\, and a comprehensive molecular and spatial analysis of the TJ-associated polarity network is lacking. In this talk I will present our recent efforts in defining the organization of the apical polarity proteins Par3 and Pals1 in fully polarized MDCK-II cells using APEX2-mediated quantitative proximity proteomics (QPP)\, electron microscopy\, and RNAi. Our data show that Pals1 defines a distinct membrane compartment apical of TJ highly reminiscent of the invertebrate marginal zone. The formation of the Pals1 domain\, which is composed of Pals1\, PatJ\, Crumbs3 and Lin7\, is strictly dependent upon Par3 expression. QPP using a Par3-Pals1 SILAC pair resolved the molecular and spatial organisation of the apical-lateral border and links the Pals1 domain to the HIPPO pathway\, GPCR signaling\, and apical membrane trafficking regulators. Finally\, prompted by our proteomics analysis we have identified a novel TJ-associated Rac1 GAP\, ARHGAP12\, which is recruited to TJ via Par3 and ZO2. Our current data suggest a model in which ARHGAP12 controls TJ formation and junctional tension via the actin polymerization factors N-WASP and WAVE2. Taken together our work defines the spatial and molecular organisation of the apical-lateral membrane border in mammalian epithelia\, uncovers an intriguing spatial conservation of invertebrate and vertebrate cell polarity proteins\, and provides a comprehensive resource for the identification of potentially novel regulators of cell polarity and mammalian cell junctions.
URL:https://www.mbi.nus.edu.sg/event/mbiw-spatially-restricted-proteomics-of-the-polarity-proteins-par3-and-pals1-reveals-sub-compartmentalization-of-the-epithelial-apical-lateral-membrane-border-by-alexander-ludwig/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Weekly Seminar
END:VEVENT
END:VCALENDAR