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PRODID:-//Mechanobiology Institute, National University of Singapore - ECPv6.15.12.2//NONSGML v1.0//EN
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X-ORIGINAL-URL:https://www.mbi.nus.edu.sg
X-WR-CALDESC:Events for Mechanobiology Institute, National University of Singapore
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BEGIN:VTIMEZONE
TZID:Asia/Shanghai
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TZOFFSETFROM:+0800
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TZNAME:CST
DTSTART:20170101T000000
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TZID:Asia/Singapore
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TZOFFSETFROM:+0800
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DTSTART:20170101T000000
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BEGIN:VEVENT
DTSTART;VALUE=DATE:20180802
DTEND;VALUE=DATE:20180812
DTSTAMP:20260426T104028
CREATED:20180525T032215Z
LAST-MODIFIED:20180727T035808Z
UID:22942-1533168000-1534031999@www.mbi.nus.edu.sg
SUMMARY:2018 Bootcamp on Mechanobiology
DESCRIPTION:The Bootcamp lectures are open to all interested graduate students and post-docs. For graduate students the lectures will be reinforced in thematic-based practicals\, leading to 4-MC. \nDates: 2 August to 11 August 2018\nWhere: CREATE Theatrette\, Level 2\, 1 CREATE Way\, CREATE Tower\, NUS \n  \nSpeakers\n\n\n\nProf Michael SHEETZ\nAssoc Prof Christoph WINKLER\nProf Peter SO\nspace\nAsst Prof CARRASCO\nDr Heidi CONNAHS\nDr Stuti Kaivalya DESAI\nAdj Assoc Prof Gregory JEDD\nAssoc Prof Pakorn Tony KANCHANAWONG\nDr Victor KOSTYUCHENKO\nAsst Prof LAU On Sun\nDr LIEU Zi Zhao\nAsst Prof LOH Ne-Te\, Duane\nProf Paul MATSUDAIRA\nAsst Prof Fumio MOTEGI\nDr ONG Lee Ling Sharon\nAsst Prof Timothy SAUNDERS\nAssoc Prof Yusuke TOYAMA\nAssoc Prof WANG Hongyan\nProf Thorsten WOHLAND\nProf YAN Jie\nAssoc Prof YEONG Foong May\nProf YU Hanry\n\n\n\n  \nProgramme Schedule and Registration\nThere are two types of programme schedule: \n\nOpen lectures & practical (the full-day programme is strictly for Year 1 NUS graduate students who would like to read MB5104 as graded module\, leading to 4-MC)\nOpen lectures\n\n\n\n\nOpen Lectures & Practical\n\n\nFOR\nREGISTER\nSCHEDULE\n\n\n• NUS graduate (Master/PhD) students (strictly for Jan & Aug 2018 intake only) \nNote: This group of students will attend the full programme and read MB5104 as a graded module (leading to 4-MC). \nThis module cannot be taken as Satisfactory or Unsatisfactory (S/U) grade.\nNUS graduate students must register through their hosting department using the prescribed template. \n Download template \nDepartment administrators should compile and send the student’s details using the prescribed template to mbicsy@nus.edu.sg  and mbical@nus.edu.sg not later than Friday\, 20th July\, 2018. \nRegistration deadline\nMonday\, 16 July\, 2018 \n\n  \n Download schedule\n\n\n\n  \n\n\n\nOpen Lectures\n\n\nFOR\nREGISTER\nSCHEDULE\n\n\n• NUS graduate students who would like to attend Bootcamp as a non-graded module (i.e.\, as a listener) \n• Non-NUS graduate students \n• Postdocs \n• Individuals who would like to know more about Mechanobiology \nNote: This group of participants will attend the open lectures only.\nNo registration required\n  \n Download schedule\n\n\n\n  \nA confirmation email will be sent to the successful students by 25 July 2018\, Wednesday. If you did not receive it by then\, please contact mbicsy@nus.edu.sg.\n\nFor more information\, contact: \nCoordinators\nAssoc Prof Yusuke Toyama (DBS)\nAsst Prof Timothy Saunders (MBI)\nDr Sharon Ong Lee Ling (SMART) \nSecretariat\nMs Carol Cheng (MBI)\nMs Chua Ai Leng (MBI) \n  \nSponsors\nThe Bootcamp 2018: An integrative Approach to Understand Cell Function gratefully acknowledges sponsorship by:
URL:https://www.mbi.nus.edu.sg/event/2018-bootcamp-on-mechanobiology/
LOCATION:CREATE Tower\, NUS University Town\, 1 Create Way\, Singapore\, 138602\, Singapore
CATEGORIES:MBI Bootcamp on Mechanobiology
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20180808T160000
DTEND;TZID=Asia/Singapore:20180808T170000
DTSTAMP:20260426T104028
CREATED:20180719T023702Z
LAST-MODIFIED:20180719T024129Z
UID:23644-1533744000-1533747600@www.mbi.nus.edu.sg
SUMMARY:Rationally designed synthetic protein hydrogels with predictable mechanical properties by Prof Cao Yi
DESCRIPTION:We are pleased to inform you on the seminar by Prof Cao Yi\, hosted by Prof Yan Jie “Rationally designed synthetic protein hydrogels with predictable mechanical properties” on 8 Aug (Wed) @ 4pm at T-Lab Level 5 Seminar Rooms.
URL:https://www.mbi.nus.edu.sg/event/rationally-designed-synthetic-protein-hydrogels-with-predictable-mechanical-properties-by-prof-cao-yi/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20180810T093000
DTEND;TZID=Asia/Singapore:20180810T103000
DTSTAMP:20260426T104028
CREATED:20180807T040945Z
LAST-MODIFIED:20181005T073303Z
UID:23928-1533893400-1533897000@www.mbi.nus.edu.sg
SUMMARY:MBIw: Numerical modeling and simulations for motility of cell populations by Prof Tetsuya Hiraiwa
DESCRIPTION:MBI Weekly Meeting Seminar\nTime: 9.30am-10.30am\nDate: Friday\, 10 August 2018\nVenue: Level 5 Seminar Room\, T-Lab \nNumerical modeling and simulations for motility of cell populations\nby Prof Tetsuya Hiraiwa\, Assistant Professor\, The University of Tokyo\, Japan
URL:https://www.mbi.nus.edu.sg/event/numerical-modeling-and-simulations-for-motility-of-cell-populations-by-prof-tetsuya-hiraiwa/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar,MBI Weekly Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20180813T163000
DTEND;TZID=Asia/Singapore:20180813T173000
DTSTAMP:20260426T104028
CREATED:20180726T021243Z
LAST-MODIFIED:20180726T021243Z
UID:23787-1534177800-1534181400@www.mbi.nus.edu.sg
SUMMARY:Visualizing the accessible genome: nanoscale in situ mapping of accessible chromatin using ATAC-see and single-molecule super-resolution fluorescence microscopy by Maurice Y Lee
DESCRIPTION:We are pleased to inform you on the seminar of Maurice Y. Lee\, hosted by Prof Pakorn Tony Kanchanawong “Visualizing the accessible genome: nanoscale in situ mapping of accessible chromatin using ATAC-see and single-molecule super-resolution fluorescence microscopy” on 13 August (Monday) @ 4.30pm at T-Lab Level 5 Seminar Rooms.
URL:https://www.mbi.nus.edu.sg/event/visualizing-the-accessible-genome-nanoscale-in-situ-mapping-of-accessible-chromatin-using-atac-see-and-single-molecule-super-resolution-fluorescence-microscopy-by-maurice-y-lee/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20180814T093000
DTEND;TZID=Asia/Singapore:20180814T103000
DTSTAMP:20260426T104028
CREATED:20180813T010628Z
LAST-MODIFIED:20181005T073618Z
UID:23935-1534239000-1534242600@www.mbi.nus.edu.sg
SUMMARY:MBI SPSS: Actin dynamics and force generation in stress fibers by Shi Shidong and Recent studies in interferometric palm system by Wang Wei
DESCRIPTION:MBI Student and Postdoc Seminar Series (SPSS)\nTime: 9.30am-10.30am\nDate: Tuesday\, 14 August\, 2018\nVenue: Level 5 Seminar Room\, T-Lab \nActin dynamics and force generation in stress fibers\nby Shi Shidong\, PhD Student\, Bershadsky Group and Viasnoff Group \n\nRecent studies in interferometric palm system\nby Wang Wei\, Research Fellow\, Kanchanawong Group \n  \n* Please note this event is open to MBI researchers and invited guests only. Thank you *
URL:https://www.mbi.nus.edu.sg/event/actin-dynamics-and-force-generation-in-stress-fibers-by-shi-shidong-and-recent-studies-in-interferometric-palm-system-by-wang-wei/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Student & Postdoc Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20180817T093000
DTEND;TZID=Asia/Singapore:20180817T103000
DTSTAMP:20260426T104028
CREATED:20180813T015624Z
LAST-MODIFIED:20181005T073508Z
UID:23954-1534498200-1534501800@www.mbi.nus.edu.sg
SUMMARY:MBIw: Signaling by WW domain-containing proteins in mechanobiology and human diseases by Prof Marius Sudol
DESCRIPTION:MBI Weekly Meeting Seminar\nTime: 9.30am-10.30am\nDate: Friday\, 17 August 2018\nVenue: Level 5 Seminar Room\, T-Lab \nSignaling by WW domain-containing proteins in mechanobiology and human diseases\nby Prof. Marius Sudol\, Co-Principal Investigator\, Mechanobiology Institute \n 
URL:https://www.mbi.nus.edu.sg/event/signaling-by-ww-domain-containing-proteins-in-mechanobiology-and-human-diseases-by-prof-marius-sudol/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar,MBI Weekly Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20180817T100000
DTEND;TZID=Asia/Singapore:20180817T120000
DTSTAMP:20260426T104028
CREATED:20180813T005957Z
LAST-MODIFIED:20180813T005957Z
UID:23931-1534500000-1534507200@www.mbi.nus.edu.sg
SUMMARY:MBI welcomes students from Konan University
DESCRIPTION:Date: 17th August\, Friday\nTime: 10.00-12.00PM (with a lecture at L10 Foyer from 10.05-10.45AM)\nPax: 35 students and 3 Faculty Members \nLab demonstrations: estimated to be from 10.45-11.45AM\n– Epi Fluorescence Microscopy (L10 Delta Vision System)\n– NSIM Superresolution System (L9 microscopy room 6)\n– Microfabrication (L9 uFab core wet bench)\n– Magnetic Tweezers (L10\, next to AFM Room) \nTherefore\, we would like to request your kind cooperation to plan your experiment/discussion with this in mind to allow for smooth progress of the lab demos from 10.45-11.45AM\, as well as the short lecture at L10 Foyer from 10.05-10.45AM.
URL:https://www.mbi.nus.edu.sg/event/mbi-welcomes-students-from-konan-university/
LOCATION:National University of Singapore
CATEGORIES:Outreach Event
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20180818T100000
DTEND;TZID=Asia/Singapore:20180818T110000
DTSTAMP:20260426T104028
CREATED:20180817T020046Z
LAST-MODIFIED:20180817T020113Z
UID:24043-1534586400-1534590000@www.mbi.nus.edu.sg
SUMMARY:Singapore Science Festival 2018: Mechanobiology: How do small cells create large living creatures? by Prof Mike Sheetz
DESCRIPTION:
URL:https://www.mbi.nus.edu.sg/event/singapore-science-festival-2018-mechanobiology-how-do-small-cells-create-large-living-creatures-by-prof-mike-sheetz/
LOCATION:Dept of Biological Sciences Seminar Room 2\, Block S2 L4\, DBS\, NUS\, Singapore
CATEGORIES:DBS Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20180820T160000
DTEND;TZID=Asia/Singapore:20180820T170000
DTSTAMP:20260426T104028
CREATED:20180816T065312Z
LAST-MODIFIED:20180816T065312Z
UID:24038-1534780800-1534784400@www.mbi.nus.edu.sg
SUMMARY:Borg proteins in melanoma cell invasion and metastasis by Aaron Farrugia
DESCRIPTION:We are pleased to inform you on the seminar by Aaron Farrugia (post-doc candidate)\, hosted by Prof Alexander Bershadsky “Borg proteins in melanoma cell invasion and metastasis” on 20 Aug (Mon) @ 4pm at T-Lab Level 5 Seminar Rooms.
URL:https://www.mbi.nus.edu.sg/event/borg-proteins-in-melanoma-cell-invasion-and-metastasis-by-aaron-farrugia/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20180821T093000
DTEND;TZID=Asia/Singapore:20180821T103000
DTSTAMP:20260426T104028
CREATED:20180815T090450Z
LAST-MODIFIED:20181005T074034Z
UID:23966-1534843800-1534847400@www.mbi.nus.edu.sg
SUMMARY:MBI SPSS: Engineering chimeric E cadherin based adherens junction by Aditya Arora
DESCRIPTION:MBI Student and Postdoc Seminar Series (SPSS)\nTime: 9.30am-10.30am\nDate: Tuesday\, 21 August\, 2018\nVenue: Level 5 Seminar Room\, T-Lab \nEngineering chimeric E cadherin based adherens junction\nby Aditya Arora\, Research Fellow\, Viasnoff Group \n* Please note this event is open to MBI researchers and invited guests only. Thank you *
URL:https://www.mbi.nus.edu.sg/event/engineering-chimeric-e-cadherin-based-adherens-junction-by-aditya-arora/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Student & Postdoc Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20180823T150000
DTEND;TZID=Asia/Singapore:20180823T160000
DTSTAMP:20260426T104028
CREATED:20180815T041706Z
LAST-MODIFIED:20180817T083815Z
UID:23956-1535036400-1535040000@www.mbi.nus.edu.sg
SUMMARY:Single-molecule analysis of transcription-coupled repair by Prof Terence Strick
DESCRIPTION:Kindly note that the following seminar is being rescheduled to 3pm. \nWe are pleased to inform you on the seminar by Prof Terence Strick\, hosted by Prof Yan Jie “Single-molecule analysis of transcription-coupled repair” on 23 Aug (Thur)  at T-Lab Level 5 Seminar Rooms.
URL:https://www.mbi.nus.edu.sg/event/single-molecule-analysis-of-transcription-coupled-repair-by-prof-terence-strick/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20180824T093000
DTEND;TZID=Asia/Singapore:20180824T103000
DTSTAMP:20260426T104028
CREATED:20180820T031119Z
LAST-MODIFIED:20181005T073550Z
UID:24050-1535103000-1535106600@www.mbi.nus.edu.sg
SUMMARY:MBIw: Elementary contractile unit and other challenging problems at the Physics Biology interface by Prof Jacques Prost
DESCRIPTION:MBI Weekly Meeting Seminar\nTime: 9.30am-10.30am\nDate: Friday\, 24 August 2018\nVenue: Level 5 Seminar Room\, T-Lab \nElementary contractile unit and other challenging problems at the Physics Biology interface\nby Prof. Jacques Prost\, Visiting Faculty\, Co-Principal Investigator\, Mechanobiology Institute\, Emeritus Professor\, Institut Curie
URL:https://www.mbi.nus.edu.sg/event/elementary-contractile-unit-and-other-challenging-problems-at-the-physics-biology-interface-by-prof-jacques-prost/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Weekly Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20180827T160000
DTEND;TZID=Asia/Singapore:20180827T170000
DTSTAMP:20260426T104028
CREATED:20180821T091417Z
LAST-MODIFIED:20180821T091417Z
UID:24110-1535385600-1535389200@www.mbi.nus.edu.sg
SUMMARY:The Study of Swelling Kinetics of Polyelectrolyte (PE) Gels by Swati Sen
DESCRIPTION:We are pleased to inform you on the seminar by Swati Sen (post-doc candidate)\, hosted by Prof Jacques Prost “The Study of Swelling Kinetics of Polyelectrolyte (PE) Gels” on 27 Aug (Mon) @ 4pm at T-Lab Level 5 Seminar Rooms. \n \n 
URL:https://www.mbi.nus.edu.sg/event/the-study-of-swelling-kinetics-of-polyelectrolyte-pe-gels-by-swati-sen/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20180828T093000
DTEND;TZID=Asia/Singapore:20180828T103000
DTSTAMP:20260426T104028
CREATED:20180824T032401Z
LAST-MODIFIED:20181005T074017Z
UID:24113-1535448600-1535452200@www.mbi.nus.edu.sg
SUMMARY:MBI SPSS: Revisit Pif1 helicase at single molecular level: Duo mode of translocation and interaction with G-quadruplex revealed by Lu Chen
DESCRIPTION:MBI Student and Postdoc Seminar Series (SPSS)\nTime: 9.30am-10.30am\nDate: Tuesday\, 28 August\, 2018\nVenue: Level 5 Seminar Room\, T-Lab \nRevisit Pif1 helicase at single molecular level: Duo mode of translocation and interaction with G-quadruplex revealed\nBy Lu Chen\, PhD Student\, Yan Jie Group \n\n* Please note this event is open to MBI researchers and invited guests only. Thank you * \n 
URL:https://www.mbi.nus.edu.sg/event/revisit-pif1-helicase-at-single-molecular-level-duo-mode-of-translocation-and-interaction-with-g-quadruplex-revealed-by-lu-chen/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Student & Postdoc Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20180831T093000
DTEND;TZID=Asia/Singapore:20180831T103000
DTSTAMP:20260426T104028
CREATED:20180827T034215Z
LAST-MODIFIED:20181005T073117Z
UID:24139-1535707800-1535711400@www.mbi.nus.edu.sg
SUMMARY:MBIw: EpCAM\, a key regulator of tissue biomechanics\, acts as a cortical organizer of cell contractility By Dr Delacour Delphine
DESCRIPTION:MBI Weekly Meeting Seminar\nTime: 9.30am-10.30am\nDate: Friday\, 31 August 2018\nVenue: Level 5 Seminar Room\, T-Lab \nEpCAM\, a key regulator of tissue biomechanics\, acts as a cortical organizer of cell contractility\nBy Dr. Delacour Delphine\, CNRS Researcher\, Institut Jacques Monod\, France \nIn monolayered epithelia\, differentiated cells are polarized and display a specific apico-basal organization.  To maintain cell polarization as well as cohesion of neighboring cells within the epithelial monolayer\, cells developed diverse adhesion complexes. However\, the epithelial monolayer is subjected to remodeling during organism development or tissue repair. In addition\, cell renewal\, cell rearrangements and cell extrusion occur to maintain the monolayer homeostasis. Consequently\, the epithelium has to constantly maintain its compartmentalization and to collectively adapt to its microenvironment to maintain its polarized and cohesive state. In recent years\, accumulating experimental evidences have led to recognition that mechanical properties of tissues have the power of directing a variety of cell functions including stem cell proliferation\, cell migration and differentiation. Various reports argue in favor of a participation of physical properties of the substrate\, such as substrate rigidity\, in the cell response for regulation of the epithelial monolayer integrity. Very little is known about the influence of the epithelial tissue geometry per se. \nOur aim is to study the influence of the tissue geometry on epithelial organization and to determine the regulatory mechanisms. These questions are addressed by developing integrated approaches at the cell biological\, biochemical and biophysical levels. As a tissue model\, we use the intestinal epithelium\, which constitutes a very good model to approach these different questions. It has a simple and regular finger-like architecture\, where proliferative and differentiate cells are distributed in distinct areas\, crypts and villi\, respectively. We mainly focus on the involvement of a protein candidate: EpCAM (Epithelial Cell Adhesion Molecule). Specifically expressed in monolayered epithelia in physiological conditions\, EpCAM was among the first discovered cancer markers in the 1970s\, and changes in its expression induce severe perturbation of epithelial tissue arrangement. Direct evidence of EpCAM involvement in epithelial morphogenesis came from a clinical study where mutations in EPCAM and subsequent loss of EpCAM expression have been correlated with the development of a rare infantile enteropathy\, the CTE (Congenital Tufting Enteropathy). The CTE intestinal epithelium displays unique morphological abnormalities\, materialized by formation of aberrant focal stacks of pseudo-multilayered epithelial cells\, named “tufts”\, making it an appealing pathological model that we use to understand the mechanisms of actions of EpCAM. \nUsing an intestinal cell line and 3D biomimetic substrates that recapitulate intestinal physical constraints\, we showed that “tuft”-like structures appear when EpCAM-deprived cells are specifically grown on 3D synthetic villi\, testifying of an enhanced mechanical stress provided by the particular topography of the monolayer substrate. EpCAM-silenced cells cultured on 3D synthetic villi can thus phenocopy CTE cellular and tissue defects. Moreover\, EpCAM loss-of-function abrogates polarized monolayer arrangement and a spatial perturbation of actomyosin occurs on villus-like 3D substrates. The alteration of epithelial contractile homeostasis is directly correlated to the development of tissue lesions\, since tissue-scale defects could be erased with contractility inhibitors. Data consequently tend to support a direct implication of EpCAM in the epithelial response to physical properties of the microenvironment. \nIn intestinal cell line and mouse and human intestinal biopsies\, we scrutinize the requirement of EpCAM for correct tissue organization and homeostasis. First\, we have showed that the absence of EpCAM provokes an unusual apico-basal polarity defects in mutant monolayers\, which stem from inappropriate actomyosin activity at tricellular junctions. Second\, we test EpCAM’s participation in early epithelial cell-substrate adhesion process and consequences on cell migration. The absence of EpCAM impairs proper single cell spreading on collagen-coated substrates with deleterious impact on front-rear polarity and migratory behaviour. In addition to a decreased protrusive activity\, the loss of EpCAM changes the morphometry of focal adhesions and abrogates proper stress fibre maturation. These modifications stem from a dysfunction of actomyosin regulation and a blocking of activated Rho zone at the cell cortex. In fact\, EpCAM acts upstream of the actomyosin apparatus and directly potentializes activated Rho recycling within the protrusion for efficient epithelial cell spreading. In parallel\, we investigate EpCAM’s participation in the homeostasis of the intestinal tissue. Within the proliferative compartment of mouse and human intestines\, EpCAM is enriched in bottom crypts\, more precisely at stem cell basolateral side\, suggesting its specific requirement for stem cell-substrate and cell-cell adhesions. Moreover\, the absence of EpCAM in CTE patients leads to clear disorganization of stem cell arrangement. Experiments pursued by using mouse EpCAM-KO 3D organoid primary cultures or CTE 3D organoid primary cultures demonstrate that EpCAM expression is required for correct de novo growth and maintenance of the intestinal stem cell niche. In-progress combination of organoids and optimized biomimetic 3D culture systems will allow more systematic analyses and live cell imaging of the intestinal niche and the differentiated epithelium along intestinal architecture. \nAltogether\, our work reveals that EpCAM acts as a key regulator of epithelial morphogenesis and homeostasis along tissue architecture by regulating actomyosin activity and actin cytoskeleton arrangement at the cell cortex.
URL:https://www.mbi.nus.edu.sg/event/epcam-a-key-regulator-of-tissue-biomechanics-acts-as-a-cortical-organizer-of-cell-contractility-by-dr-delacour-delphine/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Weekly Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20180831T110000
DTEND;TZID=Asia/Singapore:20180831T120000
DTSTAMP:20260426T104028
CREATED:20180830T012349Z
LAST-MODIFIED:20180830T012349Z
UID:24191-1535713200-1535716800@www.mbi.nus.edu.sg
SUMMARY:MBI-BIGHEART Joint Symposium: Matrix Nanotopography as a Regulator of Cell and Tissue Function by Prof Deok-Ho Kim
DESCRIPTION:
URL:https://www.mbi.nus.edu.sg/event/mbi-bigheart-joint-symposium-matrix-nanotopography-as-a-regulator-of-cell-and-tissue-function-by-prof-deok-ho-kim/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:Biomedical Institute for Global Health Research & Technology (BIGHEART),MBI Seminar
END:VEVENT
END:VCALENDAR