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X-ORIGINAL-URL:https://www.mbi.nus.edu.sg
X-WR-CALDESC:Events for Mechanobiology Institute, National University of Singapore
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DTSTART:20180101T000000
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BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20191206T100000
DTEND;TZID=Asia/Singapore:20191206T110000
DTSTAMP:20260418T053632
CREATED:20191202T060540Z
LAST-MODIFIED:20191202T060540Z
UID:27915-1575626400-1575630000@www.mbi.nus.edu.sg
SUMMARY:MBIw: Single Molecule Force Spectroscopy Reveals the Mechanical Design Governing the Efficient Translocation of the Bacterial Toxin Protein RTX by Prof Hongbin Li
DESCRIPTION:MBI Weekly Meeting Seminar\nTime: 10.00am -11.00am\nDate: Friday\, 6 Dec 2019\nVenue: Level 5 Meeting Room 1\, T-Lab \nSingle Molecule Force Spectroscopy Reveals the Mechanical Design Governing the Efficient Translocation of the Bacterial Toxin Protein RTX\nBy Prof Hongbin Li\, Department of Chemistry\, University of British Columbia\, Vancouver\, BC V6T 1Z1\, Canada (hosted by Prof. Yan Jie ) \n  \nThe efficient translocation of bacterial toxin adenylate cyclase toxin (CyaA) from the bacterial cytosol to the extracellular environment by the type 1 secretion system (T1SS) is essential for the toxin to function. To understand the molecular features that are responsible for the efficient translocation of CyaA\, here we used optical tweezers to investigate the mechanical properties and conformational dynamics of the RTX domain of CyaA at the single molecule level. Our results revealed that apo-RTX behaves like an ideal random coil. This property allows the T1SS to translocate RTX without overcoming enthalpic resistance. In contrast\, the folded holo-RTX is mechancially stable\, and its folding occurs in a vectorial\, co-translocational fashion starting from its C-terminus. Moreover\, our results showed that the folding of holo-RTX generates a stretching force\, i.e. power stroke\, which can further facilitate the translocation of RTX. Our results suggest a quasi-power-stroke mechanism for the translocation of RTX and provide mechanistic insights into the mechanical design that governs the efficient translocation of RTX.
URL:https://www.mbi.nus.edu.sg/event/mbiw-single-molecule-force-spectroscopy-reveals-the-mechanical-design-governing-the-efficient-translocation-of-the-bacterial-toxin-protein-rtx-by-prof-hongbin-li/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Weekly Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20191213T100000
DTEND;TZID=Asia/Singapore:20191213T110000
DTSTAMP:20260418T053632
CREATED:20191210T051315Z
LAST-MODIFIED:20191210T051315Z
UID:28111-1576231200-1576234800@www.mbi.nus.edu.sg
SUMMARY:MBIw: Collective and Individual Migration after the Epithelial-Mesenchymal Transition by Ian Y. Wong
DESCRIPTION:MBI Weekly Meeting Seminar\nTime: 10.00am -11.00am\nDate: Friday\, 13 Dec 2019\nVenue: Level 5 Meeting Room 1\, T-Lab \nCollective and Individual Migration after the Epithelial-Mesenchymal Transition\nBy Prof. Ian Y. Wong\, Assistant Professor\, Brown University (hosted by Prof. Lim Chwee Teck) \nCollective behaviors emerge from coordinated cell–cell interactions during the morphogenesis of tissues and tumors. For instance\, cells may display density-dependent phase transitions from a fluid-like “unjammed” phase to a solid-like “jammed” phase\, while different cell types can “self-sort”. We use comprehensive single cell tracking to elucidate these spatially and temporally heterogeneous behaviors in the context of self-organizing patterns. First\, we consider co-cultured mixtures of sheet-forming epithelial cells and dispersed mesenchymal cells\, which show a composition-dependent “unjamming” transition. Second\, we consider a gelation-like mechanism whereby cells at very sub confluent densities organize into spanning network architectures. Finally\, we analyze the disorganization and dissemination of cells cultured in 3D matrix\, which exhibit both collective and individual invasion phenotypes with distinct topological and traction signatures. These complex behaviors exhibit striking analogies with non-living systems\, suggesting that these physical concepts may be applicable to understand development and disease.
URL:https://www.mbi.nus.edu.sg/event/mbiw-collective-and-individual-migration-after-the-epithelial-mesenchymal-transition-by-ian-y-wong/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Weekly Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20191227T100000
DTEND;TZID=Asia/Singapore:20191227T110000
DTSTAMP:20260418T053632
CREATED:20191223T052437Z
LAST-MODIFIED:20191223T052437Z
UID:28303-1577440800-1577444400@www.mbi.nus.edu.sg
SUMMARY:MBIw: Potential Application of Mechanobiology in Cancer Diagnosis by Jian Yu Rao
DESCRIPTION:MBI Weekly Meeting Seminar\nTime: 10.00am -11.00am\nDate: Friday\, 27 Dec 2019\nVenue: Level 5 Meeting Room 1\, T-Lab \nPotential Application of Mechanobiology in Cancer Diagnosis\nBy Prof. Jian Yu Rao\,  Professor\, University of California\, Los Angeles\, USA (hosted by Prof. Lim Chwee Teck) \n 
URL:https://www.mbi.nus.edu.sg/event/mbiw-potential-application-of-mechanobiology-in-cancer-diagnosis-by-jian-yu-rao/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Weekly Seminar
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