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X-WR-CALNAME:Mechanobiology Institute, National University of Singapore
X-ORIGINAL-URL:https://www.mbi.nus.edu.sg
X-WR-CALDESC:Events for Mechanobiology Institute, National University of Singapore
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TZID:Asia/Singapore
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DTSTART:20180101T000000
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BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190301T093000
DTEND;TZID=Asia/Singapore:20190301T103000
DTSTAMP:20260421T104034
CREATED:20190226T030715Z
LAST-MODIFIED:20190226T030715Z
UID:25973-1551432600-1551436200@www.mbi.nus.edu.sg
SUMMARY:Re-engineering Focal Adhesions by Tony Kanchanawong
DESCRIPTION:MBI Weekly Meeting Seminar\nTime: 9.30am-10.30am\nDate: Friday\, 1 March 2019\nVenue: Level 5 Seminar Room\, T-Lab \nRe-engineering Focal Adhesions\nby Prof. Pakorn Tony Kanchanawong\, Associate Professor\, Mechanobiology Institute\, National University of Singapore.
URL:https://www.mbi.nus.edu.sg/event/re-engineering-focal-adhesions-by-tony-kanchanawong/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Weekly Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190308T093000
DTEND;TZID=Asia/Singapore:20190308T103000
DTSTAMP:20260421T104034
CREATED:20190306T065851Z
LAST-MODIFIED:20190306T065851Z
UID:26006-1552037400-1552041000@www.mbi.nus.edu.sg
SUMMARY:MBIw: Imaging Synapses: From Molecule to Function by Jun Nishiyama\, Duke-NUS Medical School
DESCRIPTION:MBI Weekly Meeting Seminar\nTime: 9.30am-10.30am\nDate: Friday\, 8 March 2019\nVenue: Level 5 Seminar Room\, T-Lab \nImaging Synapses: From Molecule to Function\nby Prof. Jun Nishiyama\, Assistant Professor\, Duke-NUS Medical School \nOur brain functions depend on connections between billions of neurons. These connections or synapses are believed to be disrupted in many neuropsychiatric disorders such as autism\, schizophrenia\, and Alzheimer’s disease. However\, molecular mechanisms underlying synaptic function and dysfunction are largely unknown. In this seminar\, I will introduce our studies to probe endogenous proteins and image signal transduction in single synapses using cutting-edge genome editing and various optical techniques. I will discuss how these tools can help to understand molecular regulation of synapses. \nReferences\n1) Nishiyama\, J.*\, Mikuni\, T*\,†.\, Yasuda R†. Virus-Mediated Genome Editing via Homology-Directed Repair in Mitotic and Postmitotic Cells in Mammalian Brain. Neuron\, 96\, 755-768 (2017). \n2) Mikuni\, T.*\, Nishiyama\, J.*\,†\, Sun Y.\, Kamasawa N.\, Yasuda\, R.† High-Throughput\, High-Resolution Mapping of Protein Localization in Mammalian Brain by In Vivo Genome Editing. Cell\, 165 1803-17 (2016). \n3) Nishiyama\, J. and Yasuda\, R. Biochemical Computation for Spine Structural Plasticity. Neuron\, 87 63-75 (2015).
URL:https://www.mbi.nus.edu.sg/event/mbiw-imaging-synapses-from-molecule-to-function-by-jun-nishiyama-duke-nus-medical-school/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Weekly Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190315T093000
DTEND;TZID=Asia/Singapore:20190315T103000
DTSTAMP:20260421T104034
CREATED:20190312T014518Z
LAST-MODIFIED:20190312T014518Z
UID:26056-1552642200-1552645800@www.mbi.nus.edu.sg
SUMMARY:MBIw: The Last Seminar: A retrospective on my past 5 years at MBI and in Singapore\, and a synopsis of my 40 years of academic research: Three items of friendly advice for young scholars by Marius Sudol
DESCRIPTION:MBI Weekly Meeting Seminar\nTime: 9.30am-10.30am\nDate: Friday\, 15 March 2019\nVenue: Level 5 Seminar Room\, T-Lab \nThe Last Seminar: A retrospective on my past 5 years at MBI and in Singapore\, and a synopsis of my 40 years of academic research: Three items of friendly advice for young scholars\nby Prof. Marius Sudol\, Co-PI at Mechanobiology Institute and Associate Professor at Department of Physiology\, National University of Singapore.
URL:https://www.mbi.nus.edu.sg/event/mbiw-the-last-seminar-a-retrospective-on-my-past-5-years-at-mbi-and-in-singapore-and-a-synopsis-of-my-40-years-of-academic-research-three-items-of-friendly-advice-for-young-scholars-by-marius-sudo/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Weekly Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Singapore:20190329T093000
DTEND;TZID=Asia/Singapore:20190329T103000
DTSTAMP:20260421T104034
CREATED:20190326T074933Z
LAST-MODIFIED:20190326T074933Z
UID:26226-1553851800-1553855400@www.mbi.nus.edu.sg
SUMMARY:MBIw: Spatially restricted proteomics of the polarity proteins Par3 and Pals1 reveals sub-compartmentalization of the epithelial apical-lateral membrane border by Alexander Ludwig
DESCRIPTION:MBI Weekly Meeting Seminar\nTime: 9.30am-10.30am\nDate: Friday\, 29 March 2019\nVenue: Level 5 Seminar Room\, T-Lab \nSpatially restricted proteomics of the polarity proteins Par3 and Pals1 reveals sub-compartmentalization of the epithelial apical-lateral membrane border\nBy Alexander Ludwig\, Assistant Professor\, School of Biological Sciences and Nanyang Institute of Structural Biology\, Nanyang Technological University\, Singapore \nIn mammalian epithelial cells the Par\, Crumbs and Scribble polarity modules occupy distinct apical and lateral membrane compartments that overlap at the level of tight junctions (TJ). However\, the precise sub-junctional organization of the polarity proteins remains unclear\, and a comprehensive molecular and spatial analysis of the TJ-associated polarity network is lacking. In this talk I will present our recent efforts in defining the organization of the apical polarity proteins Par3 and Pals1 in fully polarized MDCK-II cells using APEX2-mediated quantitative proximity proteomics (QPP)\, electron microscopy\, and RNAi. Our data show that Pals1 defines a distinct membrane compartment apical of TJ highly reminiscent of the invertebrate marginal zone. The formation of the Pals1 domain\, which is composed of Pals1\, PatJ\, Crumbs3 and Lin7\, is strictly dependent upon Par3 expression. QPP using a Par3-Pals1 SILAC pair resolved the molecular and spatial organisation of the apical-lateral border and links the Pals1 domain to the HIPPO pathway\, GPCR signaling\, and apical membrane trafficking regulators. Finally\, prompted by our proteomics analysis we have identified a novel TJ-associated Rac1 GAP\, ARHGAP12\, which is recruited to TJ via Par3 and ZO2. Our current data suggest a model in which ARHGAP12 controls TJ formation and junctional tension via the actin polymerization factors N-WASP and WAVE2. Taken together our work defines the spatial and molecular organisation of the apical-lateral membrane border in mammalian epithelia\, uncovers an intriguing spatial conservation of invertebrate and vertebrate cell polarity proteins\, and provides a comprehensive resource for the identification of potentially novel regulators of cell polarity and mammalian cell junctions.
URL:https://www.mbi.nus.edu.sg/event/mbiw-spatially-restricted-proteomics-of-the-polarity-proteins-par3-and-pals1-reveals-sub-compartmentalization-of-the-epithelial-apical-lateral-membrane-border-by-alexander-ludwig/
LOCATION:MBI Seminar Room Lvl 5\, T-Lab\, Level 5\, 5A Engineering Drive 1\, Mechanobiology Institute\, National University of Singapore\, 117411\, Singapore
CATEGORIES:MBI Weekly Seminar
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